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Antiplatelet Aggregation and Antithrombosis Efficiency of Peptides in the Snake Venom of Deinagkistrodon acutus: Isolation, Identification, and Evaluation
Joint Authors
Jiang, Fu-Sheng
Ding, Xing-Hong
Qian, Chao-Dong
Ding, Bin
Ding, Zhi-Shan
Xu, Zhenghong
Ruan, Yeping
Fan, Yongsheng
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-21
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Two peptides of Pt-A (Glu-Asn-Trp 429 Da) and Pt-B (Glu-Gln-Trp 443 Da) were isolated from venom liquor of Deinagkistrodon acutus.
Their antiplatelet aggregation effects were evaluated with platelet-rich human plasma in vitro; the respective IC50 of Pt-A and Pt-B was 66 μM and 203 μM.
Both peptides exhibited protection effects on ADP-induced paralysis in mice.
After ADP administration, the paralysis time of different concentration of Pt-A and Pt-B lasted as the following: 80 mg/kg Pt-B (152.8 ± 57.8 s) < 40 mg/kg Pt-A (163.5 ± 59.8 s) < 20 mg/kg Pt-A (253.5 ± 74.5 s) < 4 mg/kg clopidogrel (a positive control, 254.5 ± 41.97 s) < 40 mg/kg Pt-B (400.8 ± 35.9 s) < 10 mg/kg Pt-A (422.8 ± 55.4 s), all of which were statistically shorter than the saline treatment (666 ± 28 s).
Pulmonary tissue biopsy confirmed that Pt-A and Pt-B prevented the formation of thrombi in the lung.
Unlike ADP injection alone, which caused significant reduction of peripheral platelet count, Pt-A treatment prevented the drop of peripheral platelet counts; interestingly, Pt-B could not, even though the same amount of Pt-B also showed protection effects on ADP-induced paralysis and thrombosis.
More importantly, intravenous injection of Pt-A and Pt-B did not significantly increase the hemorrhage risks as clopidogrel.
American Psychological Association (APA)
Ding, Bin& Xu, Zhenghong& Qian, Chao-Dong& Jiang, Fu-Sheng& Ding, Xing-Hong& Ruan, Yeping…[et al.]. 2015. Antiplatelet Aggregation and Antithrombosis Efficiency of Peptides in the Snake Venom of Deinagkistrodon acutus: Isolation, Identification, and Evaluation. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1061475
Modern Language Association (MLA)
Ding, Bin…[et al.]. Antiplatelet Aggregation and Antithrombosis Efficiency of Peptides in the Snake Venom of Deinagkistrodon acutus: Isolation, Identification, and Evaluation. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1061475
American Medical Association (AMA)
Ding, Bin& Xu, Zhenghong& Qian, Chao-Dong& Jiang, Fu-Sheng& Ding, Xing-Hong& Ruan, Yeping…[et al.]. Antiplatelet Aggregation and Antithrombosis Efficiency of Peptides in the Snake Venom of Deinagkistrodon acutus: Isolation, Identification, and Evaluation. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1061475
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061475