Molecular Signatures in the Prevention of Radiation Damage by the Synergistic Effect of N-Acetyl Cysteine and Qingre Liyan Decoction, a Traditional Chinese Medicine, Using a 3-Dimensional Cell Culture Model of Oral Mucositis
Joint Authors
Huang, Ying
Chow, Moses Sing Sum
Lambros, Maria P.
Kondapalli, Lavanya
Parsa, Cyrus
Mulamalla, Hari Chandana
Orlando, Robert
Pon, Doreen
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Qingre Liyan decoction (QYD), a Traditional Chinese medicine, and N-acetyl cysteine (NAC) have been used to prevent radiation induced mucositis.
This work evaluates the protective mechanisms of QYD, NAC, and their combination (NAC-QYD) at the cellular and transcriptional level.
A validated organotypic model of oral mucosal consisting of a three-dimensional (3D) cell tissue-culture of primary human keratinocytes exposed to X-ray irradiation was used.
Six hours after the irradiation, the tissues were evaluated by hematoxylin and eosin (H and E) and a TUNEL assay to assess histopathology and apoptosis, respectively.
Total RNA was extracted and used for microarray gene expression profiling.
The tissue-cultures treated with NAC-QYD preserved their integrity and showed no apoptosis.
Microarray results revealed that the NAC-QYD caused the upregulation of genes encoding metallothioneins, HMOX1, and other components of the Nrf2 pathway, which protects against oxidative stress.
DNA repair genes (XCP, GADD45G, RAD9, and XRCC1), protective genes (EGFR and PPARD), and genes of the NFκB pathway were upregulated.
Finally, tissue-cultures treated prophylactically with NAC-QYD showed significant downregulation of apoptosis, cytokines and chemokines genes, and constrained damage-associated molecular patterns (DAMPs).
NAC-QYD treatment involves the protective effect of Nrf2, NFκB, and DNA repair factors.
American Psychological Association (APA)
Lambros, Maria P.& Kondapalli, Lavanya& Parsa, Cyrus& Mulamalla, Hari Chandana& Orlando, Robert& Pon, Doreen…[et al.]. 2015. Molecular Signatures in the Prevention of Radiation Damage by the Synergistic Effect of N-Acetyl Cysteine and Qingre Liyan Decoction, a Traditional Chinese Medicine, Using a 3-Dimensional Cell Culture Model of Oral Mucositis. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1061490
Modern Language Association (MLA)
Lambros, Maria P.…[et al.]. Molecular Signatures in the Prevention of Radiation Damage by the Synergistic Effect of N-Acetyl Cysteine and Qingre Liyan Decoction, a Traditional Chinese Medicine, Using a 3-Dimensional Cell Culture Model of Oral Mucositis. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1061490
American Medical Association (AMA)
Lambros, Maria P.& Kondapalli, Lavanya& Parsa, Cyrus& Mulamalla, Hari Chandana& Orlando, Robert& Pon, Doreen…[et al.]. Molecular Signatures in the Prevention of Radiation Damage by the Synergistic Effect of N-Acetyl Cysteine and Qingre Liyan Decoction, a Traditional Chinese Medicine, Using a 3-Dimensional Cell Culture Model of Oral Mucositis. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1061490
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061490