Scutellarin Reduces Endothelium Dysfunction through the PKG-I Pathway
Joint Authors
Chen, Chen
Yang, Weimin
Du, Xiaohua
Zhang, Min
Cai, Donghua
Sun, Jiaqi
Yang, Jian
Hu, Na
Ma, Congji
Zhang, Liyan
Zhang, Jun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Purpose.
In this report, we investigated the protective mechanism of scutellarin (SCU) in vitro and in vivo which could be involved in endothelial cGMP-dependent protein kinase (PKG), vasodilator stimulated phosphoprotein (VASP) pathway, and vascular endothelium dysfunction (EtD).
Method.
Human brain microvascular endothelial cells (HBMECs) with hypoxia reoxygenation (HR) treatment and rats with cerebral ischemia reperfusion (CIR) treatment were applied.
Protein and mRNA expression of PKG, VASP, and p-VASP were evaluated by Western blot and RT-PCR methods.
Vascular EtD was assessed by using wire myography to determine endothelium-dependent vasorelaxation in isolated rat basilar artery (BA).
Result.
In cultured HBMECs, SCU (0.1, 1, and 10 μM) increased cell viability, mRNA, protein level, and phosphorylative activity of PKG and VASP against HR injury.
In HR model of BA, SCU increased protein level of P-VASP.
In rat CIR model, wire myography demonstrated that SCU (45 and 90 mg/kg, i.v.) significantly reduced ischemic size by partially restoring the endothelium dependent vasodilation of BA; PKG inhibitor Rp-8-Br-cGMPS (50 μg/kg, i.v.) reversed this protection of SCU in CIR rats.
Conclusion.
SCU protects against cerebral vascular EtD through endothelial PKG pathway activation.
American Psychological Association (APA)
Du, Xiaohua& Chen, Chen& Zhang, Min& Cai, Donghua& Sun, Jiaqi& Yang, Jian…[et al.]. 2015. Scutellarin Reduces Endothelium Dysfunction through the PKG-I Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1061498
Modern Language Association (MLA)
Du, Xiaohua…[et al.]. Scutellarin Reduces Endothelium Dysfunction through the PKG-I Pathway. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1061498
American Medical Association (AMA)
Du, Xiaohua& Chen, Chen& Zhang, Min& Cai, Donghua& Sun, Jiaqi& Yang, Jian…[et al.]. Scutellarin Reduces Endothelium Dysfunction through the PKG-I Pathway. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1061498
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061498