![](/images/graphics-bg.png)
Determination of Oleanolic and Ursolic Acids in Hedyotis diffusa Using Hyphenated Ultrasound-Assisted Supercritical Carbon Dioxide Extraction and Chromatography
Joint Authors
Wei, Ming-Chi
Yang, Yu-Chiao
Hong, Show-Jen
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Oleanolic acid (OA) and ursolic acid (UA) were extracted from Hedyotis diffusa using a hyphenated procedure of ultrasound-assisted and supercritical carbon dioxide (HSC–CO2) extraction at different temperatures, pressures, cosolvent percentages, and SC–CO2 flow rates.
The results indicated that these parameters significantly affected the extraction yield.
The maximal yields of OA (0.917 mg/g of dry plant) and UA (3.540 mg/g of dry plant) were obtained at a dynamic extraction time of 110 min, a static extraction time of 15 min, 28.2 MPa, and 56°C with a 12.5% (v/v) cosolvent (ethanol/water = 82/18, v/v) and SC–CO2 flowing at 2.3 mL/min (STP).
The extracted yields were then analyzed by high performance liquid chromatography (HPLC) to quantify the OA and UA.
The present findings revealed that H.
diffusa is a potential source of OA and UA.
In addition, using the hyphenated procedure for extraction is a promising and alternative process for recovering OA and UA from H.
diffusa at high concentrations.
American Psychological Association (APA)
Wei, Ming-Chi& Yang, Yu-Chiao& Hong, Show-Jen. 2015. Determination of Oleanolic and Ursolic Acids in Hedyotis diffusa Using Hyphenated Ultrasound-Assisted Supercritical Carbon Dioxide Extraction and Chromatography. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1061507
Modern Language Association (MLA)
Wei, Ming-Chi…[et al.]. Determination of Oleanolic and Ursolic Acids in Hedyotis diffusa Using Hyphenated Ultrasound-Assisted Supercritical Carbon Dioxide Extraction and Chromatography. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1061507
American Medical Association (AMA)
Wei, Ming-Chi& Yang, Yu-Chiao& Hong, Show-Jen. Determination of Oleanolic and Ursolic Acids in Hedyotis diffusa Using Hyphenated Ultrasound-Assisted Supercritical Carbon Dioxide Extraction and Chromatography. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1061507
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061507