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Millimeter Wave Treatment Inhibits Apoptosis of Chondrocytes via Regulation Dynamic Equilibrium of Intracellular Free Ca 2+
Joint Authors
Ye, Jinxia
Wu, Guangwen
Li, Xihai
Li, Zuanfang
Zheng, Chunsong
Liu, Xianxiang
Ye, Hongzhi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The molecular mechanisms of TNF-α-induced apoptosis of chondrocyte and the role of Ca2+ mediating the effects of MW on TNF-α-induced apoptosis of chondrocytes remained unclear.
In this study, we investigated the molecular mechanism underlying inhibiting TNF-α-induced chondrocytes apoptosis of MW.
MTT assay, DAPI, and flow cytometry demonstrated that MW significantly increased cell activity and inhibited chromatin condensation accompanying the loss of plasma membrane asymmetry and the collapse of mitochondrial membrane potential.
Our results also indicated that MW reduced the elevation of [Ca2+] i in chondrocytes by LSCM.
Moreover, MW suppressed the protein levels of calpain, Bax, cytochrome c, and caspase-3, while the expressions of Bcl-2, collagen II, and aggrecan were increased.
Our evidences indicated that MW treatment inhibited the apoptosis of chondrocytes through depression of [Ca2+] i.
It also inhibited calpain activation, which mediated Bax cleavage and cytochrome c release and initiated the apoptotic execution phase.
In addition, MW treatment increased the expression of collagen II and aggrecan of chondrocytes.
American Psychological Association (APA)
Ye, Jinxia& Wu, Guangwen& Li, Xihai& Li, Zuanfang& Zheng, Chunsong& Liu, Xianxiang…[et al.]. 2015. Millimeter Wave Treatment Inhibits Apoptosis of Chondrocytes via Regulation Dynamic Equilibrium of Intracellular Free Ca 2+. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1061529
Modern Language Association (MLA)
Ye, Jinxia…[et al.]. Millimeter Wave Treatment Inhibits Apoptosis of Chondrocytes via Regulation Dynamic Equilibrium of Intracellular Free Ca 2+. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1061529
American Medical Association (AMA)
Ye, Jinxia& Wu, Guangwen& Li, Xihai& Li, Zuanfang& Zheng, Chunsong& Liu, Xianxiang…[et al.]. Millimeter Wave Treatment Inhibits Apoptosis of Chondrocytes via Regulation Dynamic Equilibrium of Intracellular Free Ca 2+. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1061529
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061529