Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism
Joint Authors
Guo, Chong-Chong
Zheng, Li-Hua
Fu, Jian-Ying
Zhu, Jian-Hong
Zhou, Yan-Xing
Zeng, Tao
Zhou, Zhi-Kun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Huangqi Sanxian decoction (HQSXD) is routinely used for the treatment of osteoporosis in the Chinese traditional healthcare system.
However, the targets and mechanism underlying the effect of HQSXD on osteoporosis have not been documented.
In the present study, seropharmacology and proteomic approaches (two-dimensional gel electrophoresis combined with mass spectrometry) were used to investigate the effects and possible target proteins of HQSXD on osteoblast.
We found that HQSXD-treated rat serum significantly enhanced osteoblast proliferation, differentiation, and mineralization.
In HQSXD-S-treated osteoblasts, there were increases in the expression of N-formyl peptide receptor 2 and heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1 and reduction in the expression of alpha-spectrin, prohibitin, and transcription elongation factor B (SIII), polypeptide 1.
The identified proteins are associated with cell proliferation, differentiation, signal transcription, and cell growth.
These findings might provide valuable insights into the mechanism of antiosteoporotic effect affected by HQSXD treatment in osteoblasts.
American Psychological Association (APA)
Guo, Chong-Chong& Zheng, Li-Hua& Fu, Jian-Ying& Zhu, Jian-Hong& Zhou, Yan-Xing& Zeng, Tao…[et al.]. 2015. Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1062406
Modern Language Association (MLA)
Guo, Chong-Chong…[et al.]. Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1062406
American Medical Association (AMA)
Guo, Chong-Chong& Zheng, Li-Hua& Fu, Jian-Ying& Zhu, Jian-Hong& Zhou, Yan-Xing& Zeng, Tao…[et al.]. Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1062406
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1062406