Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells
Joint Authors
Zhang, Xiao-Tian
Yu, Chun-Jiang
Liu, Jian-Wei
Zhang, Yan-Ping
Zhang, Chao
Song, Chen-Xue
Xie, Jing-Shu
Sai, Jing-Ying
Zheng, Jing-Tong
Wang, Fang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-05
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
We analyzed the effects of a traditional Chinese medicine, Qizhi Jiangtang Jiaonang (QJJ), on insulin resistance (IR) in vitro.
After an in vitro model of IR was established by treating human liver cancer cells (HepG2 cells) with palmitic acid, the cells were then treated with various concentrations of QJJ.
Treatment with 400 µM palmitic acid for 24 h induced IR in HepG2 cells.
The survival rate for HepG2 cells in the IR group was significantly lower than that of the untreated control group (P < 0.001); however, QJJ restored HepG2 cell survival (P < 0.001).
As compared with HepG2 cells in the IR group, QJJ at all doses analyzed significantly increased glucose consumption (all P < 0.05).
Moreover, treatment with all the QJJ doses significantly reduced the mean intracellular reactive oxygen species levels as compared with the IR group (all P < 0.05).
Furthermore, high-dose QJJ reduced both TNF-α and IL-6 levels as compared to the IR group (all P < 0.05).
QJJ ameliorated the altered PI3K, GLUT4, and RAGE expression observed with IR.
In conclusion, QJJ can improve IR in HepG2 cells, which may be mediated through the IRS-1/PI3K/GLUT4 signaling pathway as well as regulation of NF-κB-mediated inflammation and oxidative stress.
American Psychological Association (APA)
Zhang, Xiao-Tian& Yu, Chun-Jiang& Liu, Jian-Wei& Zhang, Yan-Ping& Zhang, Chao& Song, Chen-Xue…[et al.]. 2015. Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1062409
Modern Language Association (MLA)
Zhang, Xiao-Tian…[et al.]. Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1062409
American Medical Association (AMA)
Zhang, Xiao-Tian& Yu, Chun-Jiang& Liu, Jian-Wei& Zhang, Yan-Ping& Zhang, Chao& Song, Chen-Xue…[et al.]. Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1062409
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1062409