Huangzhi Oral Liquid Prevents Arrhythmias by Upregulating Caspase-3 and Apoptosis Network Proteins in Myocardial Ischemia-Reperfusion Injury in Rats
Joint Authors
Diao, Jianxin
Ran, Xu
Sun, Xue Gang
Wang, Ming
An, Hui
Huang, Guo Qiang
Zhao, Xiao Shan
Ma, Wen Xiao
Zhou, Feng Hua
Yang, Yun Gao
Miao, Can Ming
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
To study the effect of Huangzhi oral liquid (HZOL) on I/R after 2 h and 4 h and determine its regulatory function on caspase-3 and protein networks.
70 SD male rats were randomly divided into seven groups and established myocardial I/R injury model by ligating the left anterior descending coronary artery.
Myocardial infarction model was defined by TTC staining and color of the heart.
The levels of CK-MB, CTnI, C-RPL, SOD, and MDA were tested at 2 h and 4 h after reperfusion.
HE staining and ultramicrostructural were used to observe the pathological changes.
The apoptotic index (AI) of cardiomyocyte was marked by TUNEL.
The expression levels of caspase-3, p53, fas, Bcl-2, and Bax were tested by immunohistochemistry and western blot.
HZOL corrected arrhythmia, improved the pathologic abnormalities, decreased CK-MB, CTnI, C-RPL, MDA, AI, caspase-3, p53, fas, and Bax, and increased SOD ans Bcl-2 with different times of myocardial reperfusion; this result was similar to the ISMOC (P>0.05).
HZOL could inhibit arrhythmia at 2 and 4 h after I/R and ameliorate cardiac function, which was more significant at 4 h after reperfusion.
This result may be related to decreased expression of caspase-3, p53, and fas and increased Bcl-2/Bax ratio.
American Psychological Association (APA)
Ran, Xu& Diao, Jianxin& Sun, Xue Gang& Wang, Ming& An, Hui& Huang, Guo Qiang…[et al.]. 2015. Huangzhi Oral Liquid Prevents Arrhythmias by Upregulating Caspase-3 and Apoptosis Network Proteins in Myocardial Ischemia-Reperfusion Injury in Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1062410
Modern Language Association (MLA)
Ran, Xu…[et al.]. Huangzhi Oral Liquid Prevents Arrhythmias by Upregulating Caspase-3 and Apoptosis Network Proteins in Myocardial Ischemia-Reperfusion Injury in Rats. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1062410
American Medical Association (AMA)
Ran, Xu& Diao, Jianxin& Sun, Xue Gang& Wang, Ming& An, Hui& Huang, Guo Qiang…[et al.]. Huangzhi Oral Liquid Prevents Arrhythmias by Upregulating Caspase-3 and Apoptosis Network Proteins in Myocardial Ischemia-Reperfusion Injury in Rats. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1062410
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1062410