The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-Resistant Staphylococcus aureus
Joint Authors
Lee, Young-Seob
Han, Sin-Hee
Lee, Dae-Young
Kim, Yeon Bok
Lee, Sang-Won
Park, Hong-Woo
Kim, Geum-Soog
Lee, Min-Ho
Cha, Seon-Woo
Kwon, Dong-Yeul
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Shikonin (SKN), a highly liposoluble naphthoquinone pigment isolated from the roots of Lithospermum erythrorhizon, is known to exert antibacterial, wound-healing, anti-inflammatory, antithrombotic, and antitumor effects.
The aim of this study was to examine SKN antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA).
The SKN was analyzed in combination with membrane-permeabilizing agents Tris and Triton X-100, ATPase inhibitors sodium azide and N,N′-dicyclohexylcarbodiimide, and S.
aureus-derived peptidoglycan; the effects on MRSA viability were evaluated by the broth microdilution method, time-kill test, and transmission electron microscopy.
Addition of membrane-permeabilizing agents or ATPase inhibitors together with a low dose of SKN potentiated SKN anti-MRSA activity, as evidenced by the reduction of MRSA cell density by 75% compared to that observed when SKN was used alone; in contrast, addition of peptidoglycan blocked the antibacterial activity of SKN.
The results indicate that the anti-MRSA effect of SKN is associated with its affinity to peptidoglycan, the permeability of the cytoplasmic membrane, and the activity of ATP-binding cassette (ABC) transporters.
This study revealed the potential of SKN as an effective natural antibiotic and of its possible use to substantially reduce the use of existing antibiotic may also be important for understanding the mechanism underlying the antibacterial activity of natural compounds.
American Psychological Association (APA)
Lee, Young-Seob& Lee, Dae-Young& Kim, Yeon Bok& Lee, Sang-Won& Cha, Seon-Woo& Park, Hong-Woo…[et al.]. 2015. The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-Resistant Staphylococcus aureus. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1062412
Modern Language Association (MLA)
Lee, Young-Seob…[et al.]. The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-Resistant Staphylococcus aureus. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1062412
American Medical Association (AMA)
Lee, Young-Seob& Lee, Dae-Young& Kim, Yeon Bok& Lee, Sang-Won& Cha, Seon-Woo& Park, Hong-Woo…[et al.]. The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-Resistant Staphylococcus aureus. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1062412
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1062412