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Hinokitiol Negatively Regulates Immune Responses through Cell Cycle Arrest in Concanavalin A-Activated Lymphocytes
Joint Authors
Yen, Ting-Lin
Lu, Wan-Jung
Lin, Kuan-Hung
Chung, Chi-Li
Leung, Kam-Wing
He, Chia-Fu
Sheu, Joen-Rong
Lien, Li-Ming
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Autoimmune diseases are a group of chronic inflammatory diseases that arise from inappropriate inflammatory responses.
Hinokitiol, isolated from the wood of Chamaecyparis taiwanensis, engages in multiple biological activities.
Although hinokitiol has been reported to inhibit inflammation, its immunological regulation in lymphocytes remains incomplete.
Thus, we determined the effects of hinokitiol on concanavalin A- (ConA-) stimulated T lymphocytes from the spleens of mice.
In the present study, the MTT assay revealed that hinokitiol (1–5 μM) alone did not affect cell viability of lymphocytes, but at the concentration of 5 μM it could reduce ConA-stimulated T lymphocyte proliferation.
Moreover, propidium iodide (PI) staining revealed that hinokitiol arrested cell cycle of T lymphocytes at the G0/G1 phase.
Hinokitiol also reduced interferon gamma (IFN-γ) secretion from ConA-activated T lymphocytes, as detected by an ELISA assay.
In addition, hinokitiol also downregulated cyclin D3, E2F1, and Cdk4 expression and upregulated p21 expression.
These results revealed that hinokitiol may regulate immune responses.
In conclusion, we for the first time demonstrated that hinokitiol upregulates p21 expression and attenuates IFN-γ secretion in ConA-stimulated T lymphocytes, thereby arresting cell cycle at the G0/G1 phase.
In addition, our findings also indicated that hinokitiol may provide benefits to treating patients with autoimmune diseases.
American Psychological Association (APA)
Chung, Chi-Li& Leung, Kam-Wing& Lu, Wan-Jung& Yen, Ting-Lin& He, Chia-Fu& Sheu, Joen-Rong…[et al.]. 2015. Hinokitiol Negatively Regulates Immune Responses through Cell Cycle Arrest in Concanavalin A-Activated Lymphocytes. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1063364
Modern Language Association (MLA)
Chung, Chi-Li…[et al.]. Hinokitiol Negatively Regulates Immune Responses through Cell Cycle Arrest in Concanavalin A-Activated Lymphocytes. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1063364
American Medical Association (AMA)
Chung, Chi-Li& Leung, Kam-Wing& Lu, Wan-Jung& Yen, Ting-Lin& He, Chia-Fu& Sheu, Joen-Rong…[et al.]. Hinokitiol Negatively Regulates Immune Responses through Cell Cycle Arrest in Concanavalin A-Activated Lymphocytes. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1063364
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063364