Toxic Markers of Matrine Determined Using 1H-NMR-Based Metabolomics in Cultured Cells In Vitro and Rats In Vivo
Joint Authors
Zhu, Lijun
Zhang, Guiyu
Li, Zhonghuang
Zheng, Liang
Lu, Linlin
Zhang, Jiajie
Liu, Zhong-Qiu
Shi, Jian
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Matrine is one of the main bioactive alkaloids of Sophora flavescens Aiton, which has been widely used to treat various diseases in China.
These diseases include viral hepatitis, liver fibrosis, cardiac arrhythmia, skin diseases, and tumors.
However, matrine is also the main toxic compound of this herb, and the available biomarkers are not reliable in detecting or quantifying matrine risk.
Metabolomics is a powerful tool used to identify early toxicity biomarkers that are specific indicators of damage to biosystems.
This study aimed to find the potential biomarkers of the matrine-induced toxic effects in rats and HepG2 cells.
The toxicological effects of rats induced by matrine could be derived from the elevated taurine and trimethylamine N-oxide levels and the depletion in hippurate and tricarboxylic acid cycle intermediates, such as 2-oxoglutarate, citrate, and succinate in the urine.
Cell metabolomics revealed that the levels of alanine, choline, glutathione, lactate, phosphocholine, and cholesterol showed dose-dependent decreases, whereas the levels of taurine, fatty acid, and unsaturated fatty acid showed dose-dependent increases.
Overall, a significant perturbation of metabolites in response to high dose of matrine was observed both in vivo and in vitro, and the selected metabolites particularly represent an attractive marker for matrine-induced toxicity.
American Psychological Association (APA)
Li, Zhonghuang& Zheng, Liang& Shi, Jian& Zhang, Guiyu& Lu, Linlin& Zhu, Lijun…[et al.]. 2015. Toxic Markers of Matrine Determined Using 1H-NMR-Based Metabolomics in Cultured Cells In Vitro and Rats In Vivo. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1063367
Modern Language Association (MLA)
Li, Zhonghuang…[et al.]. Toxic Markers of Matrine Determined Using 1H-NMR-Based Metabolomics in Cultured Cells In Vitro and Rats In Vivo. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1063367
American Medical Association (AMA)
Li, Zhonghuang& Zheng, Liang& Shi, Jian& Zhang, Guiyu& Lu, Linlin& Zhu, Lijun…[et al.]. Toxic Markers of Matrine Determined Using 1H-NMR-Based Metabolomics in Cultured Cells In Vitro and Rats In Vivo. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1063367
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063367