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Inhibitory Effects of Chrysanthemum boreale Essential Oil on Biofilm Formation and Virulence Factor Expression of Streptococcus mutans
Joint Authors
You, Yong-Ouk
Lee, Young Eun
Kim, Beom-Su
Park, Sun-Ju
Kim, Myung-Kon
Kim, Young-Hoi
Lee, Sang-Bong
Lee, Kwang-Hee
Lee, Young-Rae
Choi, Na-Young
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-11
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The aim of the study was to evaluate the antibacterial activity of essential oil extracted from Chrysanthemum boreale (C.
boreale) on Streptococcus mutans (S.
mutans).
To investigate anticariogenic properties, and bacterial growth, acid production, biofilm formation, bacterial adherence of S.
mutans were evaluated.
Then gene expression of several virulence factors was also evaluated.
C.
boreale essential oil exhibited significant inhibition of bacterial growth, adherence capacity, and acid production of S.
mutans at concentrations 0.1–0.5 mg/mL and 0.25–0.5 mg/mL, respectively.
The safranin staining and scanning electron microscopy results showed that the biofilm formation was also inhibited.
The result of live/dead staining showed the bactericidal effect.
Furthermore, real-time PCR analysis showed that the gene expression of some virulence factors such as gtfB, gtfC, gtfD, gbpB, spaP, brpA, relA, and vicR of S.
mutans was significantly decreased in a dose dependent manner.
In GC and GC-MS analysis, seventy-two compounds were identified in the oil, representing 85.42% of the total oil.
The major components were camphor (20.89%), β-caryophyllene (5.71%), α-thujone (5.46%), piperitone (5.27%), epi-sesquiphellandrene (5.16%), α-pinene (4.97%), 1,8-cineole (4.52%), β-pinene (4.45%), and camphene (4.19%).
These results suggest that C.
boreale essential oil may inhibit growth, adhesion, acid tolerance, and biofilm formation of S.
mutans through the partial inhibition of several of these virulence factors.
American Psychological Association (APA)
Kim, Beom-Su& Park, Sun-Ju& Kim, Myung-Kon& Kim, Young-Hoi& Lee, Sang-Bong& Lee, Kwang-Hee…[et al.]. 2015. Inhibitory Effects of Chrysanthemum boreale Essential Oil on Biofilm Formation and Virulence Factor Expression of Streptococcus mutans. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1063386
Modern Language Association (MLA)
Kim, Beom-Su…[et al.]. Inhibitory Effects of Chrysanthemum boreale Essential Oil on Biofilm Formation and Virulence Factor Expression of Streptococcus mutans. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1063386
American Medical Association (AMA)
Kim, Beom-Su& Park, Sun-Ju& Kim, Myung-Kon& Kim, Young-Hoi& Lee, Sang-Bong& Lee, Kwang-Hee…[et al.]. Inhibitory Effects of Chrysanthemum boreale Essential Oil on Biofilm Formation and Virulence Factor Expression of Streptococcus mutans. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1063386
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063386