Ferulic Acid Attenuates TGF-β1-Induced Renal Cellular Fibrosis in NRK-52E Cells by Inhibiting SmadILKSnail Pathway
Joint Authors
Wei, Ming-Gang
Sun, Wei
He, Wei-ming
Ni, Li
Yang, Yan-yu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-08
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Renal fibrosis is a common cause of renal dysfunction with chronic kidney disease.
Central to this process is epithelial-mesenchymal transformation (EMT) of proximal tubular epithelial cells driven by transforming growth factor-β1 (TGF-β1) signaling.
The present study aimed to investigate the effect of Ferulic acid (FA) on EMT of renal proximal tubular epithelial cell line (NRK-52E) induced by TGF-β1 and to elucidate its underlying mechanism against EMT related to TGF-β1/Smads pathway.
The NRK-52E cells were treated for 48 h with TGF-β1 (5 ng/mL) in different concentrations of FA (0 to 200 µM).
Fibronectin, a mesenchymal marker, was assessed by western blotting.
Western blotting was also used to examine the EMT markers (E-cadherin, and α-smooth muscle actin (α-SMA)), signal transducer (p-Smad2/3), and EMT initiator (Snail).
ILK was also assayed by western blotting.
The results showed that TGF-β1 induced spindle-like morphological transition in NRK-52E cells.
Smad2/3 signaling pathway activation, increased fibronectin, α-SMA, ILK, and Snail expression, and decreased E-cadherin expression in TGF-β1-treated NRK-52E cells.
FA efficiently blocked P-Smad2/3 activation and attenuated all these EMT changes induced by TGF-β1.
These findings suggest that FA may serve as a potential fibrosis antagonist for renal proximal tubule cells by inhibiting EMT process.
American Psychological Association (APA)
Wei, Ming-Gang& Sun, Wei& He, Wei-ming& Ni, Li& Yang, Yan-yu. 2015. Ferulic Acid Attenuates TGF-β1-Induced Renal Cellular Fibrosis in NRK-52E Cells by Inhibiting SmadILKSnail Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1063389
Modern Language Association (MLA)
Wei, Ming-Gang…[et al.]. Ferulic Acid Attenuates TGF-β1-Induced Renal Cellular Fibrosis in NRK-52E Cells by Inhibiting SmadILKSnail Pathway. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1063389
American Medical Association (AMA)
Wei, Ming-Gang& Sun, Wei& He, Wei-ming& Ni, Li& Yang, Yan-yu. Ferulic Acid Attenuates TGF-β1-Induced Renal Cellular Fibrosis in NRK-52E Cells by Inhibiting SmadILKSnail Pathway. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1063389
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063389