Cytotoxic Effect of Coscinium fenestratum on Human Head and Neck Cancer Cell Line (HN31)
Joint Authors
Chiranthanut, Natthakarn
Potikanond, Saranyapin
Khonsung, Parirat
Teekachunhatean, Supanimit
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Coscinium fenestratum is widely used as a medicinal plant in many Asian countries.
This study aimed to investigate the cytotoxic effect of a crude water extract of C.
fenestratum (CF extract) compared to 5-fluorouracil (5-FU) on human HN31 cell line, a metastatic squamous cell carcinoma of the pharynx.
The results revealed that cell morphology visualized under inverted light microscopy was changed from flat with a polygonal appearance to round appearance after CF extract application.
The cell viability assay (MTT test) showed that the concentration producing 50% growth inhibition (IC50) at 48-hour incubation of CF extract on HN31 was 0.12 mg/mL, while the IC50 of 5-FU was 6.6 mg/mL, indicating that CF extract has a higher potency.
However, combining various concentrations of 5-FU and CF extract at IC50 did not show synergistic effect.
The CF extract dose dependently increased cell apoptosis determined by Annexin-V and propidium iodide staining.
It decreased the phosphorylation of p38 MAPK and pAkt, while it increased the tumor suppressor protein p53.
In conclusion, the cytotoxicity of CF extract was associated with the modulation of p38 MAPK, pAkt, and p53 signal molecules, leading to inhibiting cell survival and increasing apoptosis.
No synergistic effects of CF extract and 5-FU were observed.
American Psychological Association (APA)
Potikanond, Saranyapin& Chiranthanut, Natthakarn& Khonsung, Parirat& Teekachunhatean, Supanimit. 2015. Cytotoxic Effect of Coscinium fenestratum on Human Head and Neck Cancer Cell Line (HN31). Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1063476
Modern Language Association (MLA)
Potikanond, Saranyapin…[et al.]. Cytotoxic Effect of Coscinium fenestratum on Human Head and Neck Cancer Cell Line (HN31). Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1063476
American Medical Association (AMA)
Potikanond, Saranyapin& Chiranthanut, Natthakarn& Khonsung, Parirat& Teekachunhatean, Supanimit. Cytotoxic Effect of Coscinium fenestratum on Human Head and Neck Cancer Cell Line (HN31). Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1063476
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063476