Actinobacteria from Termite Mounds Show Antiviral Activity against Bovine Viral Diarrhea Virus, a Surrogate Model for Hepatitis C Virus
Joint Authors
Padilla, Marina Aiello
Rodrigues, Rodney Alexandre Ferreira
Bastos, Juliana Cristina Santiago
Martini, Matheus Cavalheiro
Barnabé, Ana Caroline de Souza
Kohn, Luciana Konecny
Bomfim, Getúlio Freitas
Afonso, Rafael Sanches
Fantinatti-Garboggini, Fabiana
Arns, Clarice Weis
Uetanabaro, Ana Paula Trovatti
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Extracts from termite-associated bacteria were evaluated for in vitro antiviral activity against bovine viral diarrhea virus (BVDV).
Two bacterial strains were identified as active, with percentages of inhibition (IP) equal to 98%.
Both strains were subjected to functional analysis via the addition of virus and extract at different time points in cell culture; the results showed that they were effective as posttreatments.
Moreover, we performed MTT colorimetric assays to identify the CC50, IC50, and SI values of these strains, and strain CDPA27 was considered the most promising.
In parallel, the isolates were identified as Streptomyces through 16S rRNA gene sequencing analysis.
Specifically, CDPA27 was identified as S.
chartreusis.
The CDPA27 extract was fractionated on a C18-E SPE cartridge, and the fractions were reevaluated.
A 100% methanol fraction was identified to contain the compound(s) responsible for antiviral activity, which had an SI of 262.41.
GC-MS analysis showed that this activity was likely associated with the compound(s) that had a peak retention time of 5 min.
Taken together, the results of the present study provide new information for antiviral research using natural sources, demonstrate the antiviral potential of Streptomyces chartreusis compounds isolated from termite mounds against BVDV, and lay the foundation for further studies on the treatment of HCV infection.
American Psychological Association (APA)
Padilla, Marina Aiello& Rodrigues, Rodney Alexandre Ferreira& Bastos, Juliana Cristina Santiago& Martini, Matheus Cavalheiro& Barnabé, Ana Caroline de Souza& Kohn, Luciana Konecny…[et al.]. 2015. Actinobacteria from Termite Mounds Show Antiviral Activity against Bovine Viral Diarrhea Virus, a Surrogate Model for Hepatitis C Virus. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063547
Modern Language Association (MLA)
Padilla, Marina Aiello…[et al.]. Actinobacteria from Termite Mounds Show Antiviral Activity against Bovine Viral Diarrhea Virus, a Surrogate Model for Hepatitis C Virus. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1063547
American Medical Association (AMA)
Padilla, Marina Aiello& Rodrigues, Rodney Alexandre Ferreira& Bastos, Juliana Cristina Santiago& Martini, Matheus Cavalheiro& Barnabé, Ana Caroline de Souza& Kohn, Luciana Konecny…[et al.]. Actinobacteria from Termite Mounds Show Antiviral Activity against Bovine Viral Diarrhea Virus, a Surrogate Model for Hepatitis C Virus. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063547
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063547