Effects of Selected Physicochemical Parameters on Zerumbone Production of Zingiber zerumbet Smith Cell Suspension Culture
Joint Authors
Khalid, Norzulaani
Annuar, Mohd Suffian Mohd
Tan, Boon Chin
Jalil, Mahanom
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Zingiber zerumbet Smith is an important herb that contains bioactive phytomedicinal compound, zerumbone.
To enhance cell growth and production of this useful compound, we investigated the growth conditions of cell suspension culture.
Embryogenic callus generated from shoot bud was used to initiate cell suspension culture.
The highest specific growth rate of cells was recorded when it was cultured in liquid Murashige and Skoog basal medium containing 3% sucrose with pH 5.7 and incubated under continuous shaking condition of 70 rpm for 16 h light and 8 h dark cycle at 24°C.
Our results also revealed that the type of carbohydrate substrate, light regime, agitation speed, and incubation temperature could affect the production of zerumbone.
Although the zerumbone produced in this study was not abundant compared to rhizome of Z.
zerumbet, the possibility of producing zerumbone during early stage could serve as a model for subsequent improvement.
American Psychological Association (APA)
Jalil, Mahanom& Annuar, Mohd Suffian Mohd& Tan, Boon Chin& Khalid, Norzulaani. 2015. Effects of Selected Physicochemical Parameters on Zerumbone Production of Zingiber zerumbet Smith Cell Suspension Culture. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1063566
Modern Language Association (MLA)
Jalil, Mahanom…[et al.]. Effects of Selected Physicochemical Parameters on Zerumbone Production of Zingiber zerumbet Smith Cell Suspension Culture. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1063566
American Medical Association (AMA)
Jalil, Mahanom& Annuar, Mohd Suffian Mohd& Tan, Boon Chin& Khalid, Norzulaani. Effects of Selected Physicochemical Parameters on Zerumbone Production of Zingiber zerumbet Smith Cell Suspension Culture. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1063566
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063566