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Synergy and Mode of Action of Ceftazidime plus Quercetin or Luteolin on Streptococcus pyogenes
Joint Authors
Siriwong, Supatcharee
Thumanu, Kanjana
Hengpratom, Tanaporn
Eumkeb, Griangsak
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-21
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Streptococcus pyogenes causes streptococcal toxic shock syndrome.
The recommended therapy has been often failure through the interfering of beta-lactamase-producing bacteria (BLPB).
The present study was to investigate antibacterial activity, synergy, and modes of action of luteolin and quercetin using alone and plus ceftazidime against S.
pyogenes.
The MICs of ceftazidime, luteolin, and quercetin against all S.
pyogenes were 0.50, 128, and 128 µg mL−1, respectively.
A synergistic effect was exhibited on luteolin and quercetin plus ceftazidime against these strains at fractional inhibitory concentration indices 0.37 and 0.27, respectively, and was confirmed by the viable count.
These combinations increased cytoplasmic membrane (CM) permeability, caused irregular cell shape, peptidoglycan, and CM damage, and decreased nucleic acid but increased proteins in bacterial cells.
Enzyme assay demonstrated that these flavonoids had an inhibitory activity against β-lactamase.
In summary, this study provides evidence that the inhibitory mode of action of luteolin and quercetin may be mediated via three mechanisms: (1) inhibiting of peptidoglycan synthesis, (2) increasing CM permeability, and (3) decreasing nucleic acid but increasing the protein contents of bacterial cells.
So, luteolin and quercetin propose the high potential to develop adjunct to ceftazidime for the treatment of coexistence of the BLPB and S.
pyogenes infections.
American Psychological Association (APA)
Siriwong, Supatcharee& Thumanu, Kanjana& Hengpratom, Tanaporn& Eumkeb, Griangsak. 2015. Synergy and Mode of Action of Ceftazidime plus Quercetin or Luteolin on Streptococcus pyogenes. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1063568
Modern Language Association (MLA)
Siriwong, Supatcharee…[et al.]. Synergy and Mode of Action of Ceftazidime plus Quercetin or Luteolin on Streptococcus pyogenes. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1063568
American Medical Association (AMA)
Siriwong, Supatcharee& Thumanu, Kanjana& Hengpratom, Tanaporn& Eumkeb, Griangsak. Synergy and Mode of Action of Ceftazidime plus Quercetin or Luteolin on Streptococcus pyogenes. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1063568
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063568