Protective Effects of N-Acetyl-L-Cysteine in Human Oligodendrocyte Progenitor Cells and Restoration of Motor Function in Neonatal Rats with Hypoxic-Ischemic Encephalopathy
Joint Authors
Park, Dongsun
Shin, Kyungha
Choi, Ehn-Kyoung
Choi, Youngjin
Jang, Ja-Young
Kim, Jihyun
Jeong, Heon-Sang
Lee, Wooryoung
Lee, Yoon-Bok
Kim, Seung Up
Joo, Seong Soo
Kim, Yun-Bae
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
Since oligodendrocyte progenitor cells (OPCs) are the target cells of neonatal hypoxic-ischemic encephalopathy (HIE), the present study was aimed at investigating the protective effects of N-acetyl-l-cysteine (NAC), a well-known antioxidant and precursor of glutathione, in OPCs as well as in neonatal rats.
Methods.
In in vitro study, protective effects of NAC on KCN cytotoxicity in F3.Olig2 OPCs were investigated via MTT assay and apoptotic signal analysis.
In in vivo study, NAC was administered to rats with HIE induced by hypoxia-ischemia surgery at postnatal day 7, and their motor functions and white matter demyelination were analyzed.
Results.
NAC decreased KCN cytotoxicity in F3.Olig2 cells and especially suppressed apoptosis by regulating Bcl2 and p-ERK.
Administration of NAC recovered motor functions such as the using ratio of forelimb contralateral to the injured brain, locomotor activity, and rotarod performance of neonatal HIE animals.
It was also confirmed that NAC attenuated demyelination in the corpus callosum, a white matter region vulnerable to HIE.
Conclusion.
The results indicate that NAC exerts neuroprotective effects in vitro and in vivo by preserving OPCs, via regulation of antiapoptotic signaling, and that F3.Olig2 human OPCs could be a good tool for screening of candidates for demyelinating diseases.
American Psychological Association (APA)
Park, Dongsun& Shin, Kyungha& Choi, Ehn-Kyoung& Choi, Youngjin& Jang, Ja-Young& Kim, Jihyun…[et al.]. 2015. Protective Effects of N-Acetyl-L-Cysteine in Human Oligodendrocyte Progenitor Cells and Restoration of Motor Function in Neonatal Rats with Hypoxic-Ischemic Encephalopathy. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1063577
Modern Language Association (MLA)
Park, Dongsun…[et al.]. Protective Effects of N-Acetyl-L-Cysteine in Human Oligodendrocyte Progenitor Cells and Restoration of Motor Function in Neonatal Rats with Hypoxic-Ischemic Encephalopathy. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1063577
American Medical Association (AMA)
Park, Dongsun& Shin, Kyungha& Choi, Ehn-Kyoung& Choi, Youngjin& Jang, Ja-Young& Kim, Jihyun…[et al.]. Protective Effects of N-Acetyl-L-Cysteine in Human Oligodendrocyte Progenitor Cells and Restoration of Motor Function in Neonatal Rats with Hypoxic-Ischemic Encephalopathy. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1063577
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063577