Fuyuan Decoction Enhances SOX9 and COL2A1 Expression and Smad23 Phosphorylation in IL-1β-Activated Chondrocytes
Joint Authors
Zhang, Yudi
Li, Rongheng
Zhong, Yu
Zhang, Sihan
Zhou, Lingyun
Shang, Shike
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Fuyuan Decoction (FYD), a herbal formula in China, has been widely used for osteoarthritis (OA) treatment.
Herein, we determined the effects of FYD on the expression of transcription factor SOX9 and its target gene collagen type II, alpha 1 (COL2A1) as well as the activation of Smad2/3 in interleukin- (IL-) 1β-stimulated SW1353 chondrosarcoma cells.
Serum-derived FYD (FYD-CS) was prepared to treat SW1353 cells with or without SB431542, a TGF-β1 receptor inhibitor.
Cell cycle progression was tested by flow cytometry.
The expression of SOX9 and COL2A1 and the activation of Smad2/3 (p-Smad2/3) were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and/or western blot.
The results showed that, after treatment, FYD-CS, while inducing S-phase cell cycle arrest, enhanced cell proliferation and protected the cells against IL-1β- and/or SB431542-induced cell growth inhibition.
Furthermore, FYD-CS reversed the decreased expression of COL2A1 and SOX9 induced by IL-1β and SB431542 and blocked the decreased phosphorylation of Smad2/3 induced by IL-1β alone or in combination with SB431542.
Our results suggest that FYD promotes COL2A1 and SOX9 expression as well as Smad2/3 activation in IL-1β-induced chondrocytes, thus benefiting cell survival.
American Psychological Association (APA)
Zhang, Yudi& Li, Rongheng& Zhong, Yu& Zhang, Sihan& Zhou, Lingyun& Shang, Shike. 2015. Fuyuan Decoction Enhances SOX9 and COL2A1 Expression and Smad23 Phosphorylation in IL-1β-Activated Chondrocytes. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063638
Modern Language Association (MLA)
Zhang, Yudi…[et al.]. Fuyuan Decoction Enhances SOX9 and COL2A1 Expression and Smad23 Phosphorylation in IL-1β-Activated Chondrocytes. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1063638
American Medical Association (AMA)
Zhang, Yudi& Li, Rongheng& Zhong, Yu& Zhang, Sihan& Zhou, Lingyun& Shang, Shike. Fuyuan Decoction Enhances SOX9 and COL2A1 Expression and Smad23 Phosphorylation in IL-1β-Activated Chondrocytes. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063638
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063638