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Validation of a RP-HPLC-DAD Method for Chamomile (Matricaria recutita) Preparations and Assessment of the Marker, Apigenin-7-glucoside, Safety and Anti-Inflammatory Effect
Joint Authors
Spinola, Nathália Favaretto
Ribeiro, Diego Luis
Antunes, Lusânia Maria Greggi
Hori, Juliana Issa
Berretta, Andresa Aparecida
Miguel, Felipe Galeti
Barcelos, Gustavo Rafael Mazzaron
Rocha, Bruno Alves
Marquele-Oliveira, Franciane
Cavalheiro, Amanda Henriques
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Chamomile is a medicinal plant, which presents several biological effects, especially the anti-inflammatory effect.
One of the compounds related to this effect is apigenin, a flavonoid that is mostly found in its glycosylated form, apigenin-7-glucoside (APG), in natural sources.
However, the affectivity and safety of this glycoside have not been well explored for topical application.
In this context, the aim of this work was to develop and validate a reversed-phase high-performance liquid chromatography (RP-HPLC-DAD) method to quantify APG in chamomile preparations.
Additionally, the safety and the anti-inflammatory potential of this flavonoid were verified.
The RP-HPLC-DAD method was developed and validated with linearity at 24.0–36.0 μg/mL range ( r = 0.9994 ).
Intra- and interday precision (RSD) were 0.27–2.66% and accuracy was 98.27–101.21%.
The validated method was applied in the analysis of chamomile flower heads, glycolic extract, and Kamillen cream, supporting the method application in the quality control of chamomile preparations.
Furthermore, the APG safety was assessed by MTT cytotoxicity assay and mutagenic protocols and the anti-inflammatory activity was confirmed by a diminished TNF- α production showed by mice macrophages treated with APG following LPS treatment.
American Psychological Association (APA)
Miguel, Felipe Galeti& Cavalheiro, Amanda Henriques& Spinola, Nathália Favaretto& Ribeiro, Diego Luis& Barcelos, Gustavo Rafael Mazzaron& Antunes, Lusânia Maria Greggi…[et al.]. 2015. Validation of a RP-HPLC-DAD Method for Chamomile (Matricaria recutita) Preparations and Assessment of the Marker, Apigenin-7-glucoside, Safety and Anti-Inflammatory Effect. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063661
Modern Language Association (MLA)
Miguel, Felipe Galeti…[et al.]. Validation of a RP-HPLC-DAD Method for Chamomile (Matricaria recutita) Preparations and Assessment of the Marker, Apigenin-7-glucoside, Safety and Anti-Inflammatory Effect. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1063661
American Medical Association (AMA)
Miguel, Felipe Galeti& Cavalheiro, Amanda Henriques& Spinola, Nathália Favaretto& Ribeiro, Diego Luis& Barcelos, Gustavo Rafael Mazzaron& Antunes, Lusânia Maria Greggi…[et al.]. Validation of a RP-HPLC-DAD Method for Chamomile (Matricaria recutita) Preparations and Assessment of the Marker, Apigenin-7-glucoside, Safety and Anti-Inflammatory Effect. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063661
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063661