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Pfaffosidic Fraction from Hebanthe paniculata Induces Cell Cycle Arrest and Caspase-3-Induced Apoptosis in HepG2 Cells
Joint Authors
Cogliati, Bruno
Zaidan Dagli, Maria Lúcia
da Silva, Tereza Cristina
Latorre, Andréia Oliveira
Akisue, Gokithi
Nagamine, Márcia Kazumi
Haraguchi, Mitsue
Hansen, Daiane
Sanches, Daniel Soares
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Hebanthe paniculata roots (formerly Pfaffia paniculata and popularly known as Brazilian ginseng) show antineoplastic, chemopreventive, and antiproliferative properties.
Functional properties of these roots and their extracts are usually attributed to the pfaffosidic fraction, which is composed mainly by pfaffosides A–F.
However, the therapeutic potential of this fraction in cancer cells is not yet entirely understood.
This study aimed to analyze the antitumoral effects of the purified pfaffosidic fraction or saponinic fraction on the human hepatocellular carcinoma HepG2 cell line.
Cellular viability, proliferation, and apoptosis were evaluated, respectively, by MTT assay, BrdU incorporation, activated caspase-3 immunocytochemistry, and DNA fragmentation assay.
Cell cycle was analyzed by flow cytometry and the cell cycle-related proteins were analyzed by quantitative PCR and Western blot.
The cells exposed to pfaffosidic fraction had reduced viability and cellular growth, induced G2/M at 48 h or S at 72 h arrest, and increased sub-G1 cell population via cyclin E downregulation, p27KIP1 overexpression, and caspase-3-induced apoptosis, without affecting the DNA integrity.
Antitumoral effects of pfaffosidic fraction from H.
paniculata in HepG2 cells originated by multimechanisms of action might be associated with cell cycle arrest in the S phase, by CDK2 and cyclin E downregulation and p27KIP1 overexpression, besides induction of apoptosis through caspase-3 activation.
American Psychological Association (APA)
da Silva, Tereza Cristina& Cogliati, Bruno& Latorre, Andréia Oliveira& Akisue, Gokithi& Nagamine, Márcia Kazumi& Haraguchi, Mitsue…[et al.]. 2015. Pfaffosidic Fraction from Hebanthe paniculata Induces Cell Cycle Arrest and Caspase-3-Induced Apoptosis in HepG2 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063667
Modern Language Association (MLA)
da Silva, Tereza Cristina…[et al.]. Pfaffosidic Fraction from Hebanthe paniculata Induces Cell Cycle Arrest and Caspase-3-Induced Apoptosis in HepG2 Cells. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1063667
American Medical Association (AMA)
da Silva, Tereza Cristina& Cogliati, Bruno& Latorre, Andréia Oliveira& Akisue, Gokithi& Nagamine, Márcia Kazumi& Haraguchi, Mitsue…[et al.]. Pfaffosidic Fraction from Hebanthe paniculata Induces Cell Cycle Arrest and Caspase-3-Induced Apoptosis in HepG2 Cells. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063667
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063667