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Oxidative Stress Type Influences the Properties of Antioxidants Containing Polyphenols in RINm5F Beta Cells
Joint Authors
Muller, Christian D.
Schini-Kerth, Valérie B.
Auberval, Nathalie
Dal, Stéphanie
Bietiger, William
Seyfritz, Elodie
Peluso, Jean
Zhao, Minjie
Marchioni, Eric
Pinget, Michel
Jeandidier, Nathalie
Maillard, Elisa
Sigrist, Séverine
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-05
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The in vitro methods currently used to screen bioactive compounds focus on the use of a single model of oxidative stress.
However, this simplistic view may lead to conflicting results.
The aim of this study was to evaluate the antioxidant properties of two natural extracts (a mix of red wine polyphenols (RWPs) and epigallocatechin gallate (EGCG)) with three models of oxidative stress induced with hydrogen peroxide (H2O2), a mixture of hypoxanthine and xanthine oxidase (HX/XO), or streptozotocin (STZ) in RINm5F beta cells.
We employed multiple approaches to validate their potential as therapeutic treatment options, including cell viability, reactive oxygen species production, and antioxidant enzymes expression.
All three oxidative stresses induced a decrease in cell viability and an increase in apoptosis, whereas the level of ROS production was variable depending on the type of stress.
The highest level of ROS was found for the HX/XO-induced stress, an increase that was reflected by higher expression antioxidant enzymes.
Further, both antioxidant compounds presented beneficial effects during oxidative stress, but EGCG appeared to be a more efficient antioxidant.
These data indicate that the efficiency of natural antioxidants is dependent on both the nature of the compound and the type of oxidative stress generated.
American Psychological Association (APA)
Auberval, Nathalie& Dal, Stéphanie& Bietiger, William& Seyfritz, Elodie& Peluso, Jean& Muller, Christian D.…[et al.]. 2015. Oxidative Stress Type Influences the Properties of Antioxidants Containing Polyphenols in RINm5F Beta Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1063689
Modern Language Association (MLA)
Auberval, Nathalie…[et al.]. Oxidative Stress Type Influences the Properties of Antioxidants Containing Polyphenols in RINm5F Beta Cells. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1063689
American Medical Association (AMA)
Auberval, Nathalie& Dal, Stéphanie& Bietiger, William& Seyfritz, Elodie& Peluso, Jean& Muller, Christian D.…[et al.]. Oxidative Stress Type Influences the Properties of Antioxidants Containing Polyphenols in RINm5F Beta Cells. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1063689
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063689