Chronic Treatment with a Water-Soluble Extract from the Culture Medium of Ganoderma lucidum Mycelia Prevents Apoptosis and Necroptosis in HypoxiaIschemia-Induced Injury of Type 2 Diabetic Mouse Brain
Joint Authors
Iwata, Naohiro
Xuan, Meiyan
Kamiuchi, Shinya
Matsuzaki, Hirokazu
Okazaki, Mari
Hibino, Yasuhide
Asano, Satoshi
Sakamoto, Takeshi
Miyano, Yoshiyuki
Iizuka, Hiroshi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-06
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Type 2 diabetes mellitus has been known to increase systemic oxidative stress by chronic hyperglycemia and visceral obesity and aggravate cerebral ischemic injury.
On the basis of our previous study regarding a water-soluble extract from the culture medium of Ganoderma lucidum mycelia (designed as MAK), which exerts antioxidative and neuroprotective effects, the present study was conducted to evaluate the preventive effects of MAK on apoptosis and necroptosis (a programmed necrosis) induced by hypoxia/ischemia (H/I) in type 2 diabetic KKAy mice.
H/I was induced by a combination of unilateral common carotid artery ligation with hypoxia (8% O2 for 20 min) and subsequent reoxygenation.
Pretreatment with MAK (1 g/kg, p.o.) for a week significantly reduced H/I-induced neurological deficits and brain infarction volume assessed at 24 h of reoxygenation.
Histochemical analysis showed that MAK significantly suppressed superoxide production, neuronal cell death, and vacuolation in the ischemic penumbra, which was accompanied by a decrease in the numbers of TUNEL- or cleaved caspase-3-positive cells.
Furthermore, MAK decreased the expression of receptor-interacting protein kinase 3 mRNA and protein, a key molecule for necroptosis.
These results suggest that MAK confers resistance to apoptotic and necroptotic cell death and relieves H/I-induced cerebral ischemic injury in type 2 diabetic mice.
American Psychological Association (APA)
Xuan, Meiyan& Okazaki, Mari& Iwata, Naohiro& Asano, Satoshi& Kamiuchi, Shinya& Matsuzaki, Hirokazu…[et al.]. 2015. Chronic Treatment with a Water-Soluble Extract from the Culture Medium of Ganoderma lucidum Mycelia Prevents Apoptosis and Necroptosis in HypoxiaIschemia-Induced Injury of Type 2 Diabetic Mouse Brain. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1063701
Modern Language Association (MLA)
Xuan, Meiyan…[et al.]. Chronic Treatment with a Water-Soluble Extract from the Culture Medium of Ganoderma lucidum Mycelia Prevents Apoptosis and Necroptosis in HypoxiaIschemia-Induced Injury of Type 2 Diabetic Mouse Brain. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1063701
American Medical Association (AMA)
Xuan, Meiyan& Okazaki, Mari& Iwata, Naohiro& Asano, Satoshi& Kamiuchi, Shinya& Matsuzaki, Hirokazu…[et al.]. Chronic Treatment with a Water-Soluble Extract from the Culture Medium of Ganoderma lucidum Mycelia Prevents Apoptosis and Necroptosis in HypoxiaIschemia-Induced Injury of Type 2 Diabetic Mouse Brain. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1063701
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063701