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Trigonella foenum-graceum (Seed) Extract Interferes with Quorum Sensing Regulated Traits and Biofilm Formation in the Strains of Pseudomonas aeruginosa and Aeromonas hydrophila
Joint Authors
Husain, Fohad Mabood
Shahnawaz Khan, Mohd
Al-Shabib, Nasser Abdulatif
Ahmad, Iqbal
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Trigonella foenum-graecum L.
(Fenugreek) is an important plant of the Leguminosae family known to have medicinal properties.
However, fraction based antiquorum sensing and antibiofilm activities have not been reported from this plant.
In the present study T.
foenum-graecum seed extract was sequentially fractionated and sub-MICs were tested for above activities.
The methanol fraction of the extract demonstrated significant inhibition of AHL regulated virulence factors: protease, LasB elastase, pyocyanin production, chitinase, EPS, and swarming motility in Pseudomonas aeruginosa PAO1 and PAF79.
Further, QS dependent virulence factor in the aquatic pathogen Aeromonas hydrophila WAF38 was also reduced.
Application of T.
foenum-graecum seed extract to PAO1, PAF79, and WAF38 decreased the biofilm forming abilities of the pathogens by significant levels.
The extract also exhibited reduced AHL levels and subsequent downregulation of lasB gene.
In vivo study showed an enhanced survival of PAO1-preinfected C.
elegans after treatment with extract at 1 mg/mL.
Further, the major compound detected by GC-MS, caffeine, reduced the production of QS regulated virulence factors and biofilm at 200 µg/mL concentration indicating its role in the activity of the methanol extract.
The results of the present study reveal the potential anti-QS and antibiofilm property of T.
foenum-graceum extract and caffeine.
American Psychological Association (APA)
Husain, Fohad Mabood& Ahmad, Iqbal& Shahnawaz Khan, Mohd& Al-Shabib, Nasser Abdulatif. 2015. Trigonella foenum-graceum (Seed) Extract Interferes with Quorum Sensing Regulated Traits and Biofilm Formation in the Strains of Pseudomonas aeruginosa and Aeromonas hydrophila. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1063722
Modern Language Association (MLA)
Husain, Fohad Mabood…[et al.]. Trigonella foenum-graceum (Seed) Extract Interferes with Quorum Sensing Regulated Traits and Biofilm Formation in the Strains of Pseudomonas aeruginosa and Aeromonas hydrophila. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1063722
American Medical Association (AMA)
Husain, Fohad Mabood& Ahmad, Iqbal& Shahnawaz Khan, Mohd& Al-Shabib, Nasser Abdulatif. Trigonella foenum-graceum (Seed) Extract Interferes with Quorum Sensing Regulated Traits and Biofilm Formation in the Strains of Pseudomonas aeruginosa and Aeromonas hydrophila. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1063722
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063722