Metabolomics Profiling to Investigate the Pharmacologic Mechanisms of Berberine for the Treatment of High-Fat Diet-Induced Nonalcoholic Steatohepatitis
Joint Authors
Gao, Xiaoyan
Li, Jian
Jiang, Miao
Guo, Hongtao
Lu, Aiping
Xu, Kejia
Guo, Mingxing
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
Berberine has been used to treat nonalcoholic steatohepatitis (NASH), which has been addressed in many studies.
In this study, we investigated the molecular pharmacology mechanisms of berberine using metabolomic techniques.
Methods.
Sprague-Dawley rats were randomly divided into three groups (10 rats in each group): (i) normal control group; (ii) high-fat diet- (HFD-) induced NASH model group; and (iii) HFD berberine-treated group (i.d.
200 mg/kg).
The handling procedure lasted eight weeks.
Then, UPLC-Q-TOF/MS techniques coupled with histopathology and biochemical analyses were adopted to explore the mechanisms of berberine on the protective effects against NASH.
Key Findings.
(i) According to conventional test results, berberine treatment plays a fighting role in HFD-induced NASH due to its beneficial effects against insulin resistance, inflammation, and lipid metabolism.
(ii) Based on UPLC-Q-TOF/MS techniques, metabolic profiles that involved sphingomyelin (SM), phosphatidylcholine (PC), lysophosphatidylcholine (LysoPC), 13-hydroperoxy-9, 11-octadecadienoic acid (13-HpODE), eicosatrienoic acid, docosatrienoic acid, and eicosenoic acid could provide potential metabolic biomarkers to address the pharmacological mechanisms of berberine.
Conclusions.
The parts of molecular pharmacological mechanisms of berberine for NASH treatment are related to the regulation of metabolic disruption involving phospholipid and unsaturated fatty acids in rats with NASH.
American Psychological Association (APA)
Li, Jian& Guo, Hongtao& Guo, Mingxing& Xu, Kejia& Jiang, Miao& Lu, Aiping…[et al.]. 2015. Metabolomics Profiling to Investigate the Pharmacologic Mechanisms of Berberine for the Treatment of High-Fat Diet-Induced Nonalcoholic Steatohepatitis. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063748
Modern Language Association (MLA)
Li, Jian…[et al.]. Metabolomics Profiling to Investigate the Pharmacologic Mechanisms of Berberine for the Treatment of High-Fat Diet-Induced Nonalcoholic Steatohepatitis. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1063748
American Medical Association (AMA)
Li, Jian& Guo, Hongtao& Guo, Mingxing& Xu, Kejia& Jiang, Miao& Lu, Aiping…[et al.]. Metabolomics Profiling to Investigate the Pharmacologic Mechanisms of Berberine for the Treatment of High-Fat Diet-Induced Nonalcoholic Steatohepatitis. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063748
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063748