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Antioxidant Activity and Antibacterial Effects on Clinical Isolated Streptococcus suis and Staphylococcus intermedius of Extracts from Several Parts of Cladogynos orientalis and Their Phytochemical Screenings
Joint Authors
Sithisarn, Patchima
Kongkiatpaiboon, Sumet
Sithisarn, Pongtip
Rojsanga, Piyanuch
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The in vitro antioxidant and antibacterial assays against clinically isolated Streptococcus suis and Staphylococcus intermedius of the extracts prepared by decoction and ethanolic reflux of different parts of Chettaphangki (Cladogynos orientalis Zipp.
ex Span), including the leaves, roots, and stems, using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay and disc diffusion method were conducted.
Quantitative analysis of total phenolic and total flavonoid contents in the extracts using spectrophotometric methods was also performed.
Finally, phytochemical screening by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) was conducted.
Leaf ethanolic reflux extract (100 g) contained the highest total phenolic and total flavonoid contents of 7.21 ± 0.28 μg gallic acid equivalent (GAE) and 11.51 ± 2.02 μg rutin equivalent (RE), respectively.
Chettaphangki extracts promoted low antioxidant activity with EC50 values in the range of 0.27–0.48 mg/mL.
Extracts and fractions from the roots and stems of this plant promoted low to intermediate antibacterial activity against S.
intermedius with the inhibition zones between 7 and 14 mm.
The chromatographic data suggested that the leaf extracts of C.
orientalis contained rutin while the root and stem extracts contained scopoletin and chettaphanin I.
Rutin promoted strong antioxidant activity while chettaphanin I showed low antibacterial activity against Staphylococcus intermedius.
American Psychological Association (APA)
Sithisarn, Pongtip& Rojsanga, Piyanuch& Sithisarn, Patchima& Kongkiatpaiboon, Sumet. 2015. Antioxidant Activity and Antibacterial Effects on Clinical Isolated Streptococcus suis and Staphylococcus intermedius of Extracts from Several Parts of Cladogynos orientalis and Their Phytochemical Screenings. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1063780
Modern Language Association (MLA)
Sithisarn, Pongtip…[et al.]. Antioxidant Activity and Antibacterial Effects on Clinical Isolated Streptococcus suis and Staphylococcus intermedius of Extracts from Several Parts of Cladogynos orientalis and Their Phytochemical Screenings. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1063780
American Medical Association (AMA)
Sithisarn, Pongtip& Rojsanga, Piyanuch& Sithisarn, Patchima& Kongkiatpaiboon, Sumet. Antioxidant Activity and Antibacterial Effects on Clinical Isolated Streptococcus suis and Staphylococcus intermedius of Extracts from Several Parts of Cladogynos orientalis and Their Phytochemical Screenings. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1063780
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063780