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Polypeptide Fraction from Arca subcrenata Induces Apoptosis and G2M Phase Arrest in HeLa Cells via ROS-Mediated MAPKs Pathways
Joint Authors
Zhu, Jianhua
Song, Liyan
Yu, Rongmin
Hu, Xianjing
Zhang, Zhang
Liu, Ting
Guo, Zhongyi
Cai, Jinghua
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-21
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Arca subcrenata is documented in the literature of marine Traditional Chinese Medicine.
Polypeptide fraction from A.
subcrenata, coded as P2, was demonstrated to possess significant antitumor activity in our previous study.
However, the underlying mechanism remains undefined.
The present study was carried out to investigate the underlying antitumor mechanism of P2 in human cervical cancer HeLa cells by MTT, FCM, LSCM, and western blot assays.
The results revealed that P2 significantly induced apoptosis of HeLa cells in a concentration- and time-dependent manner.
High level of ROS was provoked by P2, which was in turn responsible for induction of apoptosis through activation of intrinsic mitochondrial pathway and JNK1/2, p38 MAPK pathways, as well as inhibition of ERK1/2 pathway, as evidenced by the abrogation of P2’s effect on HeLa cells preincubated with the ROS scavenger NAC.
P2 also was observed to display significant effect on G2/M phase arrest by downregulating the expression of cyclin B1/cdc2 complex and upregulating the expression of p21.
These findings demonstrate that P2 induces apoptosis and G2/M phase arrest in HeLa cells through ROS-mediated MAPKs pathways, suggesting that P2 would be worth investigating as a promising agent within the scope of marine drugs for treatment of cervical cancer.
American Psychological Association (APA)
Hu, Xianjing& Zhang, Zhang& Liu, Ting& Song, Liyan& Zhu, Jianhua& Guo, Zhongyi…[et al.]. 2015. Polypeptide Fraction from Arca subcrenata Induces Apoptosis and G2M Phase Arrest in HeLa Cells via ROS-Mediated MAPKs Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1063816
Modern Language Association (MLA)
Hu, Xianjing…[et al.]. Polypeptide Fraction from Arca subcrenata Induces Apoptosis and G2M Phase Arrest in HeLa Cells via ROS-Mediated MAPKs Pathways. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1063816
American Medical Association (AMA)
Hu, Xianjing& Zhang, Zhang& Liu, Ting& Song, Liyan& Zhu, Jianhua& Guo, Zhongyi…[et al.]. Polypeptide Fraction from Arca subcrenata Induces Apoptosis and G2M Phase Arrest in HeLa Cells via ROS-Mediated MAPKs Pathways. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1063816
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063816