Genome-Wide Expression of MicroRNAs Is Regulated by DNA Methylation in Hepatocarcinogenesis
Joint Authors
Shen, Jing
Wang, Shuang
Siegel, Abby B.
Remotti, Helen
Wang, Qiao
Sirosh, Iryna
Santella, Regina M.
Source
Gastroenterology Research and Practice
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
Previous studies, including ours, have examined the regulation of microRNAs (miRNAs) by DNA methylation, but whether this regulation occurs at a genome-wide level in hepatocellular carcinoma (HCC) is unclear.
Subjects/Methods.
Using a two-phase study design, we conducted genome-wide screening for DNA methylation and miRNA expression to explore the potential role of methylation alterations in miRNAs regulation.
Results.
We found that expressions of 25 miRNAs were statistically significantly different between tumor and nontumor tissues and perfectly differentiated HCC tumor from nontumor.
Six miRNAs were overexpressed, and 19 were repressed in tumors.
Among 133 miRNAs with inverse correlations between methylation and expression, 8 miRNAs (6%) showed statistically significant differences in expression between tumor and nontumor tissues.
Six miRNAs were validated in 56 additional paired HCC tissues, and significant inverse correlations were observed for miR-125b and miR-199a, which is consistent with the inactive chromatin pattern found in HepG2 cells.
Conclusion.
These data suggest that the expressions of miR-125b and miR-199a are dramatically regulated by DNA hypermethylation that plays a key role in hepatocarcinogenesis.
American Psychological Association (APA)
Shen, Jing& Wang, Shuang& Siegel, Abby B.& Remotti, Helen& Wang, Qiao& Sirosh, Iryna…[et al.]. 2015. Genome-Wide Expression of MicroRNAs Is Regulated by DNA Methylation in Hepatocarcinogenesis. Gastroenterology Research and Practice،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1063936
Modern Language Association (MLA)
Shen, Jing…[et al.]. Genome-Wide Expression of MicroRNAs Is Regulated by DNA Methylation in Hepatocarcinogenesis. Gastroenterology Research and Practice No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1063936
American Medical Association (AMA)
Shen, Jing& Wang, Shuang& Siegel, Abby B.& Remotti, Helen& Wang, Qiao& Sirosh, Iryna…[et al.]. Genome-Wide Expression of MicroRNAs Is Regulated by DNA Methylation in Hepatocarcinogenesis. Gastroenterology Research and Practice. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1063936
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063936