KRAS and BRAF Mutation Detection: Is Immunohistochemistry a Possible Alternative to Molecular Biology in Colorectal Cancer?
Joint Authors
Bolognese, Antonio
Piton, Nicolas
Borrini, Francesco
Lamy, Aude
Sabourin, Jean-Christophe
Source
Gastroenterology Research and Practice
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
KRAS genotyping is mandatory in metastatic colorectal cancer treatment prior to undertaking antiepidermal growth factor receptor (EGFR) monoclonal antibody therapy.
BRAF V600E mutation is often present in colorectal carcinoma with CpG island methylator phenotype and microsatellite instability.
Currently, KRAS and BRAF evaluation is based on molecular biology techniques such as SNaPshot or Sanger sequencing.
As molecular testing is performed on formalin-fixed paraffin-embedded (FFPE) samples, immunodetection would appear to be an attractive alternative for detecting mutations.
Thus, our objective was to assess the validity of KRAS and BRAF immunodetection of mutations compared with the genotyping reference method in colorectal adenocarcinoma.
KRAS and BRAF genotyping was assessed by SNaPshot.
A rabbit anti-human KRAS polyclonal antibody was tested on 33 FFPE colorectal tumor samples with known KRAS status.
Additionally, a mouse anti-human BRAF monoclonal antibody was tested on 30 FFPE tumor samples with known BRAF status.
KRAS immunostaining demonstrated both poor sensitivity (27%) and specificity (64%) in detecting KRAS mutation.
Conversely, BRAF immunohistochemistry showed perfect sensitivity (100%) and specificity (100%) in detecting V600E mutation.
Although molecular biology remains the reference method for detecting KRAS mutation, immunohistochemistry could be an attractive method for detecting BRAF V600E mutation in colorectal cancer.
American Psychological Association (APA)
Piton, Nicolas& Borrini, Francesco& Bolognese, Antonio& Lamy, Aude& Sabourin, Jean-Christophe. 2015. KRAS and BRAF Mutation Detection: Is Immunohistochemistry a Possible Alternative to Molecular Biology in Colorectal Cancer?. Gastroenterology Research and Practice،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1064112
Modern Language Association (MLA)
Piton, Nicolas…[et al.]. KRAS and BRAF Mutation Detection: Is Immunohistochemistry a Possible Alternative to Molecular Biology in Colorectal Cancer?. Gastroenterology Research and Practice No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1064112
American Medical Association (AMA)
Piton, Nicolas& Borrini, Francesco& Bolognese, Antonio& Lamy, Aude& Sabourin, Jean-Christophe. KRAS and BRAF Mutation Detection: Is Immunohistochemistry a Possible Alternative to Molecular Biology in Colorectal Cancer?. Gastroenterology Research and Practice. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1064112
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1064112