Isolation, Characterization, and RP-HPLC Estimation of P-Coumaric Acid from Methanolic Extract of Durva Grass (Cynodon dactylon Linn.) (Pers.)
Joint Authors
Karthikeyan, Ramadoss
Devadasu, Chapala
Srinivasa Babu, Puttagunta
Source
International Journal of Analytical Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
P-coumaric acid is a nonflavonoid phenolic acid and is a major constituent of the species Cynodon dactylon Linn.
(Pers.).
In this study isolation of P-coumaric acid was achieved by preparative TLC and the compound thus isolated was characterised by UV, mass, and H1 NMR spectral analysis.
An isocratic RP-HPLC method was developed for the estimation of P-coumaric acid from methanolic extracts of durva grass.
The chromatographic separations were achieved by RP-C18 column (250 mm × 4.6 mm, 5 μ), Shimadzu LC-20AT Prominence liquid chromatograph, and a mobile phase composed of water : methanol : glacial acetic acid (65 : 34 : 1 v/v).
The flow rate was 1.0 mL/min and the analyses of column effluents were performed using UV-visible detector at 310 nm.
Retention time of P-coumaric acid was found to be 6.617 min.
This method has obeyed linearity over the concentration range of 2–10 μg/mL and the regression coefficient obtained from linearity plot for P-coumaric acid was found to be 0.999.
RP-HPLC method was validated in pursuance of ICH guidelines.
American Psychological Association (APA)
Karthikeyan, Ramadoss& Devadasu, Chapala& Srinivasa Babu, Puttagunta. 2015. Isolation, Characterization, and RP-HPLC Estimation of P-Coumaric Acid from Methanolic Extract of Durva Grass (Cynodon dactylon Linn.) (Pers.). International Journal of Analytical Chemistry،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1064408
Modern Language Association (MLA)
Karthikeyan, Ramadoss…[et al.]. Isolation, Characterization, and RP-HPLC Estimation of P-Coumaric Acid from Methanolic Extract of Durva Grass (Cynodon dactylon Linn.) (Pers.). International Journal of Analytical Chemistry No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1064408
American Medical Association (AMA)
Karthikeyan, Ramadoss& Devadasu, Chapala& Srinivasa Babu, Puttagunta. Isolation, Characterization, and RP-HPLC Estimation of P-Coumaric Acid from Methanolic Extract of Durva Grass (Cynodon dactylon Linn.) (Pers.). International Journal of Analytical Chemistry. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1064408
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1064408