Micellar Enhanced Spectrofluorimetric Method for the Determination of Ponatinib in Human Plasma and Urine via Cremophor RH 40 as Sensing Agent
Joint Authors
Abdelhameed, Ali Saber
Darwish, Hany W.
Bakheit, Ahmed H.
AlKhairallah, Amer S.
Source
International Journal of Analytical Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
An impressively simple and precise spectrofluorimetric procedure was established and validated for ponatinib (PTB) quantitation in biological fluids such as human plasma and human urine.
This method depends on examining the fluorescence characteristics of PTB in a micellar system of Cremophor RH 40 (Cr RH 40).
Cr RH 40 enhanced the intrinsic fluorescence of PTB distinctly in aqueous water.
The fluorescence spectra of PTB was recorded at 457 nm following its excitation at 305 nm.
Maximum fluorescence intensity was attained by addition of 0.7 mL of Cr RH 40 and one mL of phosphate buffer to PTB aliquots and then dilution with distilled water.
There is a linear relationship between the fluorescence intensity of PTB and its concentration over the range 5–120 ngmL−1, with limit of detection and limit of quantification equal to 0.905 ngmL−1 and 2.742 ngmL−1, respectively.
The accuracy and the precisions of the proposed method were checked and gave adequate results.
The adopted method was applied with a great success for PTB quantitation in different biological matrices (spiked human plasma and urine) giving high recovery values.
American Psychological Association (APA)
Darwish, Hany W.& Bakheit, Ahmed H.& Abdelhameed, Ali Saber& AlKhairallah, Amer S.. 2015. Micellar Enhanced Spectrofluorimetric Method for the Determination of Ponatinib in Human Plasma and Urine via Cremophor RH 40 as Sensing Agent. International Journal of Analytical Chemistry،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1064410
Modern Language Association (MLA)
Darwish, Hany W.…[et al.]. Micellar Enhanced Spectrofluorimetric Method for the Determination of Ponatinib in Human Plasma and Urine via Cremophor RH 40 as Sensing Agent. International Journal of Analytical Chemistry No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1064410
American Medical Association (AMA)
Darwish, Hany W.& Bakheit, Ahmed H.& Abdelhameed, Ali Saber& AlKhairallah, Amer S.. Micellar Enhanced Spectrofluorimetric Method for the Determination of Ponatinib in Human Plasma and Urine via Cremophor RH 40 as Sensing Agent. International Journal of Analytical Chemistry. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1064410
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1064410