Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation
Joint Authors
Amin, Imran
Jabbar, Tania
Niazi, Fawad
Akhtar, Muhammad Saeed
Source
International Journal of Analytical Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-19
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
A semiautomated extraction protocol of HCV-RNA using Favorgen RNA extraction kit has been developed.
The kit provided protocol was modified by replacing manual spin steps with vacuum filtration.
The assay performance was evaluated by real-time qPCR based on Taqman technology.
Assay linearity was confirmed with the serial dilutions of RTA (Turkey) containing 1 × (106, 105, 104, and 103) IU mL−1.
Comparison of test results obtained by two extraction methods showed a good correlation (r=0.95, n=30) with detection limit of 102 IU mL−1.
The semiautomated vacuum filtration based protocol demonstrated high throughput: 35 minutes for the extraction of a batch of 30 samples (150 µL each) with reduced labor, time, waste, and cost.
Performance characteristics of semiautomated system make it suitable for use in diagnostic purpose and viral load determinations.
American Psychological Association (APA)
Amin, Imran& Jabbar, Tania& Niazi, Fawad& Akhtar, Muhammad Saeed. 2015. Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation. International Journal of Analytical Chemistry،Vol. 2015, no. 2015, pp.1-4.
https://search.emarefa.net/detail/BIM-1064422
Modern Language Association (MLA)
Amin, Imran…[et al.]. Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation. International Journal of Analytical Chemistry No. 2015 (2015), pp.1-4.
https://search.emarefa.net/detail/BIM-1064422
American Medical Association (AMA)
Amin, Imran& Jabbar, Tania& Niazi, Fawad& Akhtar, Muhammad Saeed. Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation. International Journal of Analytical Chemistry. 2015. Vol. 2015, no. 2015, pp.1-4.
https://search.emarefa.net/detail/BIM-1064422
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1064422
