Ammonium Ion Exchanged Zeolite for Laser DesorptionIonization Mass Spectrometry of Phosphorylated Peptides

Abstract EN

α-Cyano-4-hydroxycinnamic acid (CHCA), an organic matrix molecule for matrix-assisted laser desorption/ionization mass spectrometry, was adsorbed to NH 4 + -type zeolite surface, and this new matrix was used for the detection of low-molecular-weight compounds.

It was found that this matrix could simplify the mass spectrum in the low-molecular-weight region and prevent interference from fragments and alkali metal ion adducted species.

CHCA adsorbed to NH 4 + -type ZSM5 zeolite (CHCA/NH4ZSM5) was used to measure atropine and aconitine, two toxic alkaloids in plants.

In addition, CHCA/NH4ZSM5 enabled us to detect phosphorylated peptides; peaks of the protonated peptides had higher intensities than the peaks observed using CHCA only.