Comparison of Electrospray Ionization and Atmospheric Chemical Ionization Coupled with the Liquid Chromatography-Tandem Mass Spectrometry for the Analysis of Cholesteryl Esters
Joint Authors
Lee, Hae-Rim
Kochhar, Sunil
Shim, Soon-Mi
Source
International Journal of Analytical Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-19
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The approach of two different ionization techniques including electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was tested for the analysis of cholesteryl esters (CEs).
The retention time (RT), signal intensity, protonated ion, and product ion of CEs were compared between ESI and APCI.
RT of CEs from both ionizations decreased with increasing double bonds, while it increased with longer carbon chain length.
The ESI process generated strong signal intensity of precursor ions corresponding to [M+Na]+ and [M+NH4]+ regardless of the number of carbon chains and double bonds in CEs.
On the other hand, the APCI process produced a protonated ion of CEs [M+H]+ with a weak signal intensity, and it is selectively sensitive to detect precursor ions of CEs with unsaturated fatty acids.
The ESI technique proved to be effective in ionizing more kinds of CEs than the APCI technique.
American Psychological Association (APA)
Lee, Hae-Rim& Kochhar, Sunil& Shim, Soon-Mi. 2015. Comparison of Electrospray Ionization and Atmospheric Chemical Ionization Coupled with the Liquid Chromatography-Tandem Mass Spectrometry for the Analysis of Cholesteryl Esters. International Journal of Analytical Chemistry،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1064432
Modern Language Association (MLA)
Lee, Hae-Rim…[et al.]. Comparison of Electrospray Ionization and Atmospheric Chemical Ionization Coupled with the Liquid Chromatography-Tandem Mass Spectrometry for the Analysis of Cholesteryl Esters. International Journal of Analytical Chemistry No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1064432
American Medical Association (AMA)
Lee, Hae-Rim& Kochhar, Sunil& Shim, Soon-Mi. Comparison of Electrospray Ionization and Atmospheric Chemical Ionization Coupled with the Liquid Chromatography-Tandem Mass Spectrometry for the Analysis of Cholesteryl Esters. International Journal of Analytical Chemistry. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1064432
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1064432