Stability-Indicating Assay for the Determination of Pentobarbital Sodium in Liquid Formulations
Joint Authors
Ajemni, Myriam
Balde, Issa-Bella
Kabiche, Sofiane
Carret, Sandra
Fontan, Jean-Eudes
Cisternino, Salvatore
Schlatter, Joël
Source
International Journal of Analytical Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-12
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A stability-indicating assay by reversed-phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of pentobarbital sodium in oral formulations: a drug used for infant sedation in computed tomography (CT) or magnetic resonance imaging (MRI) scan.
The chromatographic separation was achieved on a reversed-phase C18 column, using isocratic elution and a detector set at 214 nm.
The optimized mobile phase consisted of a 0.01 M potassium buffer pH 3 and methanol (40 : 60, v/v).
The flow rate was 1.0 mL/min and the run time of analysis was 5 min.
The linearity of the method was demonstrated in the range of 5 to 250 μg/mL pentobarbital sodium solution (r2 = 0.999).
The limit of detection and limit of quantification were 2.10 and 3.97 μg/mL, respectively.
The intraday and interday precisions were less than 2.1%.
Accuracy of the method ranged from 99.2 to 101.3%.
Stability studies indicate that the drug is stable to sunlight and in aqueous solution.
Accelerated pentobarbital sodium breakdown by strong alkaline, acidic, or oxidative stress produced noninterfering peaks.
This method allows accurate and reliable determination of pentobarbital sodium for drug stability assay in pharmaceutical studies.
American Psychological Association (APA)
Ajemni, Myriam& Balde, Issa-Bella& Kabiche, Sofiane& Carret, Sandra& Fontan, Jean-Eudes& Cisternino, Salvatore…[et al.]. 2015. Stability-Indicating Assay for the Determination of Pentobarbital Sodium in Liquid Formulations. International Journal of Analytical Chemistry،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1064434
Modern Language Association (MLA)
Ajemni, Myriam…[et al.]. Stability-Indicating Assay for the Determination of Pentobarbital Sodium in Liquid Formulations. International Journal of Analytical Chemistry No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1064434
American Medical Association (AMA)
Ajemni, Myriam& Balde, Issa-Bella& Kabiche, Sofiane& Carret, Sandra& Fontan, Jean-Eudes& Cisternino, Salvatore…[et al.]. Stability-Indicating Assay for the Determination of Pentobarbital Sodium in Liquid Formulations. International Journal of Analytical Chemistry. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1064434
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1064434