Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides
Joint Authors
Chen, Nai-Dong
Chen, Nai-Fu
Li, Jun
Cao, Cai-Yun
Wang, Jin-Mei
Huang, He-Ping
Source
International Journal of Analytical Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
GC-MS method combined with FTIR techniques by the analysis of polysaccharide was applied to evaluate the similarity between wild (W) and tissue-cultured (TC) Dendrobium huoshanense (DHS), Dendrobium officinale (DO), and Dendrobium moniliforme (DM) as well as 3 wild Dendrobium spp.: Dendrobium henanense (DHN), Dendrobium loddigesii (DL), and Dendrobium crepidatum (DC).
Eight monosaccharides involving xylose, arabinose, rhamnose, glucose, mannose, fructose, galactose, and galacturonic acid were identified in the polysaccharide from each Dendrobium sample while the contents of the monosugars varied remarkably across origins and species.
Further similarity evaluation based on GC-MS data showed that the rcor values of different origins of DHS, DO, and DM were 0.831, 0.865, and 0.884, respectively, while the rcor values ranged from 0.475 to 0.837 across species.
FTIR files of the polysaccharides revealed that the similarity coefficients between W and TC-DHS, DO, and DM were 88.7%, 86.8%, and 88.5%, respectively, in contrast to the similarity coefficients varying from 57.4% to 82.6% across species.
These results suggested that the structures of polysaccharides between different origins of the investigated Dendrobiums might be higher than what we had supposed.
American Psychological Association (APA)
Chen, Nai-Dong& Chen, Nai-Fu& Li, Jun& Cao, Cai-Yun& Wang, Jin-Mei& Huang, He-Ping. 2015. Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides. International Journal of Analytical Chemistry،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1064437
Modern Language Association (MLA)
Chen, Nai-Dong…[et al.]. Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides. International Journal of Analytical Chemistry No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1064437
American Medical Association (AMA)
Chen, Nai-Dong& Chen, Nai-Fu& Li, Jun& Cao, Cai-Yun& Wang, Jin-Mei& Huang, He-Ping. Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides. International Journal of Analytical Chemistry. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1064437
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1064437