Development of a HPLC Method for the Quantitative Determination of Capsaicin in Collagen Sponge
Joint Authors
Zhou, Yanfang
Guo, Chun-Lian
Chen, Hong-Ying
Cui, Bi-Ling
Chen, Yu-Huan
Wang, Qin
Peng, Xinsheng
Source
International Journal of Analytical Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-03
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Controlling the concentration of drugs in pharmaceutical products is essential to patient’s safety.
In this study, a simple and sensitive HPLC method is developed to quantitatively analyze capsaicin in collagen sponge.
The capsaicin from sponge was extracted for 30 min with ultrasonic wave extraction technique and methanol was used as solvent.
The chromatographic method was performed by using isocratic system composed of acetonitrile-water (70 : 30) with a flow rate of 1 mL/min and the detection wavelength was at 280 nm.
Capsaicin can be successfully separated with good linearity (the regression equation is A = 9.7182C + 0.8547; R2 = 1.0) and perfect recovery (99.72%).
The mean capsaicin concentration in collagen sponge was 49.32 mg/g (RSD = 1.30%; n = 3).
In conclusion, the ultrasonic wave extraction method is simple and the extracting efficiency is high.
The HPLC assay has excellent sensitivity and specificity and is a convenient method for capsaicin detection in collagen sponge.
This paper firstly discusses the quantitative analysis of capsaicin in collagen sponge.
American Psychological Association (APA)
Guo, Chun-Lian& Chen, Hong-Ying& Cui, Bi-Ling& Chen, Yu-Huan& Zhou, Yanfang& Peng, Xinsheng…[et al.]. 2015. Development of a HPLC Method for the Quantitative Determination of Capsaicin in Collagen Sponge. International Journal of Analytical Chemistry،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1064455
Modern Language Association (MLA)
Guo, Chun-Lian…[et al.]. Development of a HPLC Method for the Quantitative Determination of Capsaicin in Collagen Sponge. International Journal of Analytical Chemistry No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1064455
American Medical Association (AMA)
Guo, Chun-Lian& Chen, Hong-Ying& Cui, Bi-Ling& Chen, Yu-Huan& Zhou, Yanfang& Peng, Xinsheng…[et al.]. Development of a HPLC Method for the Quantitative Determination of Capsaicin in Collagen Sponge. International Journal of Analytical Chemistry. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1064455
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1064455