![](/images/graphics-bg.png)
Extracellular Vesicles from Caveolin-Enriched Microdomains Regulate Hyaluronan-Mediated Sustained Vascular Integrity
Joint Authors
Mirzapoiazova, Tamara
Lennon, Frances E.
Mambetsariev, Bolot
Allen, Michael
Riehm, Jacob
Poroyko, Valeriy A.
Singleton, Patrick A.
Source
International Journal of Cell Biology
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-10
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Defects in vascular integrity are an initiating factor in several disease processes.
We have previously reported that high molecular weight hyaluronan (HMW-HA), a major glycosaminoglycan in the body, promotes rapid signal transduction in human pulmonary microvascular endothelial cells (HPMVEC) leading to barrier enhancement.
In contrast, low molecular weight hyaluronan (LMW-HA), produced in disease states by hyaluronidases and reactive oxygen species (ROS), induces HPMVEC barrier disruption.
However, the mechanism(s) of sustained barrier regulation by HA are poorly defined.
Our results indicate that long-term (6–24 hours) exposure of HMW-HA induced release of a novel type of extracellular vesicle from HLMVEC called enlargeosomes (characterized by AHNAK expression) while LMW-HA long-term exposure promoted release of exosomes (characterized by CD9, CD63, and CD81 expression).
These effects were blocked by inhibiting caveolin-enriched microdomain (CEM) formation.
Further, inhibiting enlargeosome release by annexin II siRNA attenuated the sustained barrier enhancing effects of HMW-HA.
Finally, exposure of isolated enlargeosomes to HPMVEC monolayers generated barrier enhancement while exosomes led to barrier disruption.
Taken together, these results suggest that differential release of extracellular vesicles from CEM modulate the sustained HPMVEC barrier regulation by HMW-HA and LMW-HA.
HMW-HA-induced specialized enlargeosomes can be a potential therapeutic strategy for diseases involving impaired vascular integrity.
American Psychological Association (APA)
Mirzapoiazova, Tamara& Lennon, Frances E.& Mambetsariev, Bolot& Allen, Michael& Riehm, Jacob& Poroyko, Valeriy A.…[et al.]. 2015. Extracellular Vesicles from Caveolin-Enriched Microdomains Regulate Hyaluronan-Mediated Sustained Vascular Integrity. International Journal of Cell Biology،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1065326
Modern Language Association (MLA)
Mirzapoiazova, Tamara…[et al.]. Extracellular Vesicles from Caveolin-Enriched Microdomains Regulate Hyaluronan-Mediated Sustained Vascular Integrity. International Journal of Cell Biology No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1065326
American Medical Association (AMA)
Mirzapoiazova, Tamara& Lennon, Frances E.& Mambetsariev, Bolot& Allen, Michael& Riehm, Jacob& Poroyko, Valeriy A.…[et al.]. Extracellular Vesicles from Caveolin-Enriched Microdomains Regulate Hyaluronan-Mediated Sustained Vascular Integrity. International Journal of Cell Biology. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1065326
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1065326