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A Simple and Efficient Method for Preparing Cell Slides and Staining without Using Cytocentrifuge and Cytoclips
Joint Authors
Hu, Xiaotang
Laguerre, Verronika
Packert, Daniel
Nakasone, Alice
Moscinski, Lynn
Source
International Journal of Cell Biology
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-17
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Cell staining is a necessary and useful technique for visualizing cell morphology and structure under a microscope.
This technique has been used in many areas such as cytology, hematology, oncology, histology, virology, serology, microbiology, cell biology, and immunochemistry.
One of the key pieces of equipment for preparing a slide for cell staining is cytology centrifuge (cytocentrifuge) such as cytospin.
However, many small labs do not have this expensive equipment and its accessory, cytoclips (also expensive relatively), which makes them difficult to study cell cytology.
Here we present an alternative method for preparing a slide and cell staining in the absence of a cytocentrifuge (and cytoclips).
This method is based on the principle that a regular cell centrifuge can be used to concentrate cells harvested from cell culture and then deposit the concentrated cell suspension to a slide evenly by using a cell spreader, followed by cell staining.
The method presented is simple, rapid, economic, and efficient.
This method may also avoid a possible change in cell morphology induced by cytocentrifuge.
American Psychological Association (APA)
Hu, Xiaotang& Laguerre, Verronika& Packert, Daniel& Nakasone, Alice& Moscinski, Lynn. 2015. A Simple and Efficient Method for Preparing Cell Slides and Staining without Using Cytocentrifuge and Cytoclips. International Journal of Cell Biology،Vol. 2015, no. 2015, pp.1-4.
https://search.emarefa.net/detail/BIM-1065357
Modern Language Association (MLA)
Hu, Xiaotang…[et al.]. A Simple and Efficient Method for Preparing Cell Slides and Staining without Using Cytocentrifuge and Cytoclips. International Journal of Cell Biology No. 2015 (2015), pp.1-4.
https://search.emarefa.net/detail/BIM-1065357
American Medical Association (AMA)
Hu, Xiaotang& Laguerre, Verronika& Packert, Daniel& Nakasone, Alice& Moscinski, Lynn. A Simple and Efficient Method for Preparing Cell Slides and Staining without Using Cytocentrifuge and Cytoclips. International Journal of Cell Biology. 2015. Vol. 2015, no. 2015, pp.1-4.
https://search.emarefa.net/detail/BIM-1065357
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1065357