High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular Analysis
Joint Authors
Engström-Laurent, Anna
Do, Lan
Dahl, Christen P.
Kerje, Susanne
Hansell, Peter
Lindqvist, Ulla
Larsson, Göran
Hellman, Urban
Mörner, Stellan
Source
International Journal of Cell Biology
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-10
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Hyaluronan is a negatively charged polydisperse polysaccharide where both its size and tissue concentration play an important role in many physiological and pathological processes.
The various functions of hyaluronan depend on its molecular size.
Up to now, it has been difficult to study the role of hyaluronan in diseases with pathological changes in the extracellular matrix where availability is low or tissue samples are small.
Difficulty to obtain large enough biopsies from human diseased tissue or tissue from animal models has also restricted the study of hyaluronan.
In this paper, we demonstrate that gas-phase electrophoretic molecular mobility analyzer (GEMMA) can be used to estimate the distribution of hyaluronan molecular sizes in biological samples with a limited amount of hyaluronan.
The low detection level of the GEMMA method allows for estimation of hyaluronan molecular sizes from different parts of small organs.
Hence, the GEMMA method opens opportunity to attain a profile over the distribution of hyaluronan molecular sizes and estimate changes caused by disease or experimental conditions that has not been possible to obtain before.
American Psychological Association (APA)
Do, Lan& Dahl, Christen P.& Kerje, Susanne& Hansell, Peter& Mörner, Stellan& Lindqvist, Ulla…[et al.]. 2015. High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular Analysis. International Journal of Cell Biology،Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1065362
Modern Language Association (MLA)
Do, Lan…[et al.]. High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular Analysis. International Journal of Cell Biology No. 2015 (2015), pp.1-5.
https://search.emarefa.net/detail/BIM-1065362
American Medical Association (AMA)
Do, Lan& Dahl, Christen P.& Kerje, Susanne& Hansell, Peter& Mörner, Stellan& Lindqvist, Ulla…[et al.]. High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular Analysis. International Journal of Cell Biology. 2015. Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1065362
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1065362