Genetic Evaluation of E. coli Strains Isolated from Asymptomatic Children with Neurogenic Bladders
Joint Authors
Kryger, John
Burleigh, Alexandra
Christensen, Melissa
Hopkins, Walter
Source
International Journal of Chronic Diseases
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-01
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
This study was conducted to describe the genetic profiles of E.
coli that colonize asymptomatic pediatric neurogenic bladders.
E.
coli was isolated from 25 of 80 urine samples.
Patients were excluded if they presented with symptomatic urinary tract infection or received treatment with antibiotics in the preceding three months.
Multiplex PCR was performed to determine E.
coli phylotype (A, B1, B2, and D) and the presence of seven pathogenicity islands (PAIs) and 10 virulence factors (VFs).
E.
coli strains were predominantly of the B1 and B2 phylotype, with few strains in the A or D phylotype.
The PAIs IV536, ICFT073, and IICFT073 had the highest prevalence: 76%, 64%, and 48%, respectively.
The PAIs II536, IJ96, and IIJ96 were less prevalent: 28%, 20%, and 24%, respectively.
The most prevalent VF was vat (40%), while the least prevalent VFs were sfa (8%) and iha (12%).
None of the strains carried the VF fyuA, which is very common in uropathogenic E.
coli (UPEC).
The genetic profiles of E.
coli in this cohort seem to be more similar to UPEC than to commensal E.
coli.
However, they appear to have reduced virulence potential that allows them to colonize asymptomatically.
American Psychological Association (APA)
Kryger, John& Burleigh, Alexandra& Christensen, Melissa& Hopkins, Walter. 2015. Genetic Evaluation of E. coli Strains Isolated from Asymptomatic Children with Neurogenic Bladders. International Journal of Chronic Diseases،Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1065365
Modern Language Association (MLA)
Kryger, John…[et al.]. Genetic Evaluation of E. coli Strains Isolated from Asymptomatic Children with Neurogenic Bladders. International Journal of Chronic Diseases No. 2015 (2015), pp.1-5.
https://search.emarefa.net/detail/BIM-1065365
American Medical Association (AMA)
Kryger, John& Burleigh, Alexandra& Christensen, Melissa& Hopkins, Walter. Genetic Evaluation of E. coli Strains Isolated from Asymptomatic Children with Neurogenic Bladders. International Journal of Chronic Diseases. 2015. Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1065365
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1065365