Consequence of Menin Deficiency in Mouse Adipocytes Derived by In Vitro Differentiation
Joint Authors
Parekh, Vaishali I.
Modali, Sita D.
Desai, Shruti S.
Agarwal, Sunita K.
Source
International Journal of Endocrinology
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Lipoma in patients with the multiple endocrine neoplasia type 1 (MEN1) syndrome is a type of benign fat-cell tumor that has biallelic inactivation of MEN1 that encodes menin and could serve as a model to investigate normal and pathologic fat-cell (adipocyte) proliferation and function.
The role of menin and its target genes in adipocytes is not known.
We used in vitro differentiation to derive matched normal and menin-deficient adipocytes from wild type (WT) and menin-null (Men1-KO) mouse embryonic stem cells (mESCs), respectively, or 3T3-L1 cells without or with menin knockdown to investigate cell size, lipid content, and gene expression changes.
Adipocytes derived from Men1-KO mESCs or after menin knockdown in 3T3-L1 cells showed a 1.5–1.7-fold increase in fat-cell size.
Global gene expression analysis of mESC-derived adipocytes showed that lack of menin downregulated the expression of many differentially methylated genes including the tumor suppressor long noncoding RNA Meg3 but upregulated gene expression from the prolactin gene family locus.
Our results show that menin deficiency leads to fat-cell hypertrophy and provide model systems that could be used to study the regulation of fat-cell size.
American Psychological Association (APA)
Parekh, Vaishali I.& Modali, Sita D.& Desai, Shruti S.& Agarwal, Sunita K.. 2015. Consequence of Menin Deficiency in Mouse Adipocytes Derived by In Vitro Differentiation. International Journal of Endocrinology،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1065568
Modern Language Association (MLA)
Parekh, Vaishali I.…[et al.]. Consequence of Menin Deficiency in Mouse Adipocytes Derived by In Vitro Differentiation. International Journal of Endocrinology No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1065568
American Medical Association (AMA)
Parekh, Vaishali I.& Modali, Sita D.& Desai, Shruti S.& Agarwal, Sunita K.. Consequence of Menin Deficiency in Mouse Adipocytes Derived by In Vitro Differentiation. International Journal of Endocrinology. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1065568
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1065568