Methylation Status of Vitamin D Receptor Gene Promoter in Benign and Malignant Adrenal Tumors
Joint Authors
Pilon, Catia
Rebellato, Andrea
Urbanet, Riccardo
Guzzardo, Vincenza
Cappellesso, Rocco
Sasano, Hironobu
Fassina, Ambrogio
Fallo, Francesco
Source
International Journal of Endocrinology
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We previously showed a decreased expression of vitamin D receptor (VDR) mRNA/protein in a small group of adrenocortical carcinoma (ACC) tissues, suggesting the loss of a protective role of VDR against malignant cell growth in this cancer type.
Downregulation of VDR gene expression may result from epigenetics events, that is, methylation of cytosine nucleotide of CpG islands in VDR gene promoter.
We analyzed methylation of CpG sites in the VDR gene promoter in normal adrenals and adrenocortical tumor samples.
Methylation of CpG-rich 5′ regions was assessed by bisulfite sequencing PCR using bisulfite-treated DNA from archival microdissected paraffin-embedded adrenocortical tissues.
Three normal adrenals and 23 various adrenocortical tumor samples (15 adenomas and 8 carcinomas) were studied.
Methylation in the promoter region of VDR gene was found in 3/8 ACCs, while no VDR gene methylation was observed in normal adrenals and adrenocortical adenomas.
VDR mRNA and protein levels were lower in ACCs than in benign tumors, and VDR immunostaining was weak or negative in ACCs, including all 3 methylated tissue samples.
The association between VDR gene promoter methylation and reduced VDR gene expression is not a rare event in ACC, suggesting that VDR epigenetic inactivation may have a role in adrenocortical carcinogenesis.
American Psychological Association (APA)
Pilon, Catia& Rebellato, Andrea& Urbanet, Riccardo& Guzzardo, Vincenza& Cappellesso, Rocco& Sasano, Hironobu…[et al.]. 2015. Methylation Status of Vitamin D Receptor Gene Promoter in Benign and Malignant Adrenal Tumors. International Journal of Endocrinology،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1065670
Modern Language Association (MLA)
Pilon, Catia…[et al.]. Methylation Status of Vitamin D Receptor Gene Promoter in Benign and Malignant Adrenal Tumors. International Journal of Endocrinology No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1065670
American Medical Association (AMA)
Pilon, Catia& Rebellato, Andrea& Urbanet, Riccardo& Guzzardo, Vincenza& Cappellesso, Rocco& Sasano, Hironobu…[et al.]. Methylation Status of Vitamin D Receptor Gene Promoter in Benign and Malignant Adrenal Tumors. International Journal of Endocrinology. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1065670
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1065670