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Regulation of the Human Ghrelin Promoter Activity by Transcription Factors, NF-κB and Nkx2.2
Joint Authors
Shiimura, Yuki
Ohgusu, Hideko
Sato, Takahiro
Kojima, Masayasu
Source
International Journal of Endocrinology
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
To examine the gene expression of ghrelin, a growth hormone releasing and appetite stimulating hormone from stomach, we constructed human ghrelin promoter-reporter vectors and analyzed the promoter activity.
The ghrelin promoter activity was high when cultured cells that express ghrelin mRNA endogenously like TT or ECC10 cells were used, indicating that these cells contain factors necessary for full expression of the human ghrelin gene.
The human ghrelin promoter contains both positive and negative regulatory regions.
A transient decrease of the promoter activity was found when the reporter vector with the −1600 fragment of the human ghrelin promoter was transfected into cultured cells.
We then examined the effect of several transcription factors on the ghrelin promoter activity and found that NF-κB suppressed and that Nkx2.2, a homeodomain-containing transcription factor that is important for ghrelin cell development in pancreas, activates the promoter activity.
These transcription factors may be possible targets for the control of ghrelin gene expression.
American Psychological Association (APA)
Shiimura, Yuki& Ohgusu, Hideko& Sato, Takahiro& Kojima, Masayasu. 2015. Regulation of the Human Ghrelin Promoter Activity by Transcription Factors, NF-κB and Nkx2.2. International Journal of Endocrinology،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1065752
Modern Language Association (MLA)
Shiimura, Yuki…[et al.]. Regulation of the Human Ghrelin Promoter Activity by Transcription Factors, NF-κB and Nkx2.2. International Journal of Endocrinology No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1065752
American Medical Association (AMA)
Shiimura, Yuki& Ohgusu, Hideko& Sato, Takahiro& Kojima, Masayasu. Regulation of the Human Ghrelin Promoter Activity by Transcription Factors, NF-κB and Nkx2.2. International Journal of Endocrinology. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1065752
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1065752