Paraoxonase 2 Induces a Phenotypic Switch in Macrophage Polarization Favoring an M2 Anti-Inflammatory State
Joint Authors
Koren-Gluzer, Marie
Rosenblat, Mira
Hayek, Tony
Source
International Journal of Endocrinology
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Inflammatory processes are involved in atherosclerosis development.
Macrophages play a major role in the early atherogenesis, and they are present in the atherosclerotic lesion in two phenotypes: proinflammatory (M1) or anti-inflammatory (M2).
Paraoxonase 2 (PON2) is expressed in macrophages, and it was shown to protect against atherosclerosis.
Thus, the aim of our study was to analyze the direct effect of PON2 on macrophage inflammatory phenotypes.
Ex vivo studies were performed with murine peritoneal macrophages (MPM) harvested from control C57BL/6 and PON2-deficient (PON2KO) mice.
PON2KO MPM showed an enhanced proinflammatory phenotype compared to the control, both in the basal state and following M1 activation by IFNγ and lipopolysaccharide (LPS).
In parallel, PON2KO MPM also showed reduced anti-inflammatory responses in the basal state and also following M2 activation by IL-4.
Moreover, the PON2-null MPM demonstrated enhanced phagocytosis and reactive oxygen species (ROS) production in the basal state and following M1 activation.
The direct effect of PON2 was shown by transfecting human PON2 (hPON2) into PON2KO MPM.
PON2 transfection attenuated the macrophages’ response to M1 activation and enhanced M2 response.
These PON2 effects were associated with attenuation of macrophages’ abilities to phagocyte and to generate ROS.
We conclude that PON2 promotes an M1 to M2 switch in macrophage phenotypes.
American Psychological Association (APA)
Koren-Gluzer, Marie& Rosenblat, Mira& Hayek, Tony. 2015. Paraoxonase 2 Induces a Phenotypic Switch in Macrophage Polarization Favoring an M2 Anti-Inflammatory State. International Journal of Endocrinology،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1065871
Modern Language Association (MLA)
Koren-Gluzer, Marie…[et al.]. Paraoxonase 2 Induces a Phenotypic Switch in Macrophage Polarization Favoring an M2 Anti-Inflammatory State. International Journal of Endocrinology No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1065871
American Medical Association (AMA)
Koren-Gluzer, Marie& Rosenblat, Mira& Hayek, Tony. Paraoxonase 2 Induces a Phenotypic Switch in Macrophage Polarization Favoring an M2 Anti-Inflammatory State. International Journal of Endocrinology. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1065871
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1065871