Quantitative Shotgun Proteomics Analysis of Rice Anther Proteins after Exposure to High Temperature
Joint Authors
Kim, Mijeong
Kim, Hijin
Lee, Wondo
Lee, Yoonjung
Kwon, Soon-Wook
Lee, Joohyun
Source
International Journal of Genomics
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In rice, the stage of development most sensitive to high temperature stress is flowering, and exposure at this stage can result in spikelet sterility, thereby leading to significant yield losses.
In this study, protein expression patterns of rice anthers from Dianxi4, a high temperature tolerant Japonica rice variety, were compared between samples exposed to high temperature and those grown in natural field conditions in Korea.
Shotgun proteomics analysis of three replicate control and high-temperature-treated samples identified 3,266 nonredundant rice anther proteins (false discovery rate < 0.01).
We found that high levels of ATP synthase, cupin domain-containing proteins, and pollen allergen proteins were present in rice anthers.
Comparative analyses of 1,944 reproducibly expressed proteins identified 139 differentially expressed proteins, with 95 increased and 44 decreased in response to high temperature conditions.
Heat shock, DnaK family, and chaperone proteins showed highly increased expression, suggesting that the high temperature tolerance of Dianxi4 is achieved by stabilization of proteins in pollen cells.
Trehalose synthase was also highly increased after heat treatment, suggesting a possible role for trehalose in preventing protein denaturation through desiccation.
American Psychological Association (APA)
Kim, Mijeong& Kim, Hijin& Lee, Wondo& Lee, Yoonjung& Kwon, Soon-Wook& Lee, Joohyun. 2015. Quantitative Shotgun Proteomics Analysis of Rice Anther Proteins after Exposure to High Temperature. International Journal of Genomics،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1066002
Modern Language Association (MLA)
Kim, Mijeong…[et al.]. Quantitative Shotgun Proteomics Analysis of Rice Anther Proteins after Exposure to High Temperature. International Journal of Genomics No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1066002
American Medical Association (AMA)
Kim, Mijeong& Kim, Hijin& Lee, Wondo& Lee, Yoonjung& Kwon, Soon-Wook& Lee, Joohyun. Quantitative Shotgun Proteomics Analysis of Rice Anther Proteins after Exposure to High Temperature. International Journal of Genomics. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1066002
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1066002