Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.)‎

Abstract EN

Perilla frutescens is valuable as a medicinal plant as well as a natural medicine and functional food.

However, comparative genomics analyses of P.

frutescens are limited due to a lack of gene annotations and characterization.

A full-length cDNA library from P.

frutescens leaves was constructed to identify functional gene clusters and probable EST-SSR markers via analysis of 1,056 expressed sequence tags.

Unigene assembly was performed using basic local alignment search tool (BLAST) homology searches and annotated Gene Ontology (GO).

A total of 18 simple sequence repeats (SSRs) were designed as primer pairs.

This study is the first to report comparative genomics and EST-SSR markers from P.

frutescens will help gene discovery and provide an important source for functional genomics and molecular genetic research in this interesting medicinal plant.