Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism
Joint Authors
Touil-Boukoffa, Chafia
Belguendouz, Houda
Lahmar-Belguendouz, Karima
Messaoudene, Djamel
Djeraba, Zineb
Otmani, Fifi
Hakem, Djennat
Lahlou-Boukoffa, Ouided S.
Youinou, Pierre
Source
International Journal of Inflammation
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Aim and Methods.
In this study, we evaluated NOS and arginase activities and their regulation during Behçet disease, a systemic chronic inflammatory disorder with uncertain etiology.
The peripheral blood mononuclear cells of 36 patients and 15 control samples (PBMC) were cultured in either RPMI 1640, MEM, or DMEM complemented with 10% of FBS and antibiotics.
Cultures were performed with or without the control or patients plasma.
Subsequent treatment contained anticytokines (IL-6, TGF-β), a mitogenic effector (PHA), or NOS modulators (L-NMMA, BH4).
Culture supernatants were harvested after 24 h of incubation.
NO and urea measurements were, respectively, performed by modified Griess and Berthelot methods.
Results.
Higher urea levels were found in patients’ plasma compared to the control’s (P < 0.05).
NOS modulators induced inverted production profiles for NO and urea (P < 0.05).
Their results differed depending on the clinical findings (P < 0.05).
It was also found that cytokine neutralization induced different response profiles in patients as opposed to control cultures (P < 0.05).
Conclusion.
Our results suggest that arginases can compete with NOS2 for L-arginine during Behçet disease.
Both enzymes are regulated by environmental cytokines and substrate availability.
Furthermore, it seems that NOS/arginase balance is dependent on clinical expression.
American Psychological Association (APA)
Belguendouz, Houda& Lahmar-Belguendouz, Karima& Messaoudene, Djamel& Djeraba, Zineb& Otmani, Fifi& Hakem, Djennat…[et al.]. 2015. Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism. International Journal of Inflammation،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1066141
Modern Language Association (MLA)
Belguendouz, Houda…[et al.]. Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism. International Journal of Inflammation No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1066141
American Medical Association (AMA)
Belguendouz, Houda& Lahmar-Belguendouz, Karima& Messaoudene, Djamel& Djeraba, Zineb& Otmani, Fifi& Hakem, Djennat…[et al.]. Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism. International Journal of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1066141
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1066141