EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells
Joint Authors
Leng, Weiling
Lei, Xiaotian
Meng, Hao
Ouyang, Xinshou
Liang, Ziwen
Source
International Journal of Inflammation
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Our research group firstly discovered endothelial-overexpressed lipopolysaccharide-associated factor 1 (EOLA1, GenBank number AY074889) as a lipopolysaccharide (LPS) responsive gene in ECV304 cells.
The previous studies have further demonstrated the association of EOLA1 with metallothionein 2A (MT2A), while the role of EOLA1 during LPS-induced inflammatory response in ECV304 cells is unknown.
In this report, we determined the subcellular localization of EOLA1 and the regulatory capacity of EOLA1 on vascular cell adhesion molecule-1 (VCAM-1) in response to LPS in ECV304 cells.
Our results show that EOLA1 is broadly diffuse in the cells, and EOLA1 expression is dramatically induced by LPS.
EOLA1 knockdown results in significant enhancement of LPS-induced VCAM-1 production.
Consistent with this, overexpression of EOLA1 leads to the reduction of LPS-induced VCAM-1 production.
Furthermore, MT2A knockdown reduces LPS-induced VCAM-1 production.
Collectively, our results demonstrate a negative regulatory role of EOLA1 on LPS-induced VCAM-1 expression involving its association with MT2A in ECV304 cells.
American Psychological Association (APA)
Leng, Weiling& Lei, Xiaotian& Meng, Hao& Ouyang, Xinshou& Liang, Ziwen. 2015. EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells. International Journal of Inflammation،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1066142
Modern Language Association (MLA)
Leng, Weiling…[et al.]. EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells. International Journal of Inflammation No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1066142
American Medical Association (AMA)
Leng, Weiling& Lei, Xiaotian& Meng, Hao& Ouyang, Xinshou& Liang, Ziwen. EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells. International Journal of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1066142
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1066142