Rapid Accumulation of Total Lipid in Rhizoclonium africanum Kutzing as Biodiesel Feedstock under Nutrient Limitations and the Associated Changes at Cellular Level
Joint Authors
Satpati, Gour Gopal
Kanjilal, Sanjit
Narayana Prasad, Rachapudi Badari
Pal, Ruma
Source
International Journal of Microbiology
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-31
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Increase of total lipid and the proportion of the favorable fatty acids in marine green filamentous macroalga Rhizoclonium africanum (Chlorophyceae) was studied under nitrate and phosphate limitations.
These stresses were given by both eliminating and doubling the required amounts of nitrate and phosphate salts in the growth media.
A significant twofold increase in total lipid (193.03 mg/g) was achieved in cells in absence of nitrate in the culture medium, followed by phosphate limitation (142.65 mg/g).
The intracellular accumulation of neutral lipids was observed by fluorescence microscopy.
The scanning electron microscopic study showed the major structural changes under nutrient starvation.
Fourier transform infrared spectroscopy (FTIR) revealed the presence of ester (C-O-C stretching), ketone (C-C stretching), carboxylic acid (O-H bending), phosphine (P-H stretching), aromatic (C-H stretching and bending), and alcohol (O-H stretching and bending) groups in the treated cells indicating the high accumulation of lipid hydrocarbons in the treated cells.
Elevated levels of fatty acids favorable for biodiesel production, that is, C16:0, C16:1, C18:1, and C20:1, were identified under nitrate- and phosphate-deficient conditions.
This study shows that the manipulation of cultural conditions could affect the biosynthetic pathways leading to increased lipid production while increasing the proportion of fatty acids suitable for biodiesel production.
American Psychological Association (APA)
Satpati, Gour Gopal& Kanjilal, Sanjit& Narayana Prasad, Rachapudi Badari& Pal, Ruma. 2015. Rapid Accumulation of Total Lipid in Rhizoclonium africanum Kutzing as Biodiesel Feedstock under Nutrient Limitations and the Associated Changes at Cellular Level. International Journal of Microbiology،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1066167
Modern Language Association (MLA)
Satpati, Gour Gopal…[et al.]. Rapid Accumulation of Total Lipid in Rhizoclonium africanum Kutzing as Biodiesel Feedstock under Nutrient Limitations and the Associated Changes at Cellular Level. International Journal of Microbiology No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1066167
American Medical Association (AMA)
Satpati, Gour Gopal& Kanjilal, Sanjit& Narayana Prasad, Rachapudi Badari& Pal, Ruma. Rapid Accumulation of Total Lipid in Rhizoclonium africanum Kutzing as Biodiesel Feedstock under Nutrient Limitations and the Associated Changes at Cellular Level. International Journal of Microbiology. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1066167
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1066167